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The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells

OBJECTIVE(S): Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. MATERIALS AND METHODS: Isolated...

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Autores principales: Baharara, Javad, Amini, Elaheh, Kerachian, Mohammad Amin, Soltani, Mozhgan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240799/
https://www.ncbi.nlm.nih.gov/pubmed/25422758
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author Baharara, Javad
Amini, Elaheh
Kerachian, Mohammad Amin
Soltani, Mozhgan
author_facet Baharara, Javad
Amini, Elaheh
Kerachian, Mohammad Amin
Soltani, Mozhgan
author_sort Baharara, Javad
collection PubMed
description OBJECTIVE(S): Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. MATERIALS AND METHODS: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value <0.05 was considered significant. RESULTS: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis. CONCLUSION: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium.
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spelling pubmed-42407992014-11-24 The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells Baharara, Javad Amini, Elaheh Kerachian, Mohammad Amin Soltani, Mozhgan Iran J Basic Med Sci Short Communication OBJECTIVE(S): Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. MATERIALS AND METHODS: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value <0.05 was considered significant. RESULTS: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis. CONCLUSION: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium. Mashhad University of Medical Sciences 2014-08 /pmc/articles/PMC4240799/ /pubmed/25422758 Text en © Iranian Journal of Basic Medical Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Baharara, Javad
Amini, Elaheh
Kerachian, Mohammad Amin
Soltani, Mozhgan
The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
title The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
title_full The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
title_fullStr The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
title_full_unstemmed The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
title_short The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
title_sort osteogenic differentiation stimulating activity of sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240799/
https://www.ncbi.nlm.nih.gov/pubmed/25422758
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