Tissue fixation and the effect of molecular fixatives on downstream staining procedures

It is impossible to underplay the importance of fixation in histopathology. Whether the scientist is interested in the extraction of information on lipids, proteins, RNA or DNA, fixation is critical to this extraction. This review aims to give a brief overview of the current “state of play” in fixat...

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Detalles Bibliográficos
Autores principales: Howat, William J., Wilson, Beverley A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240801/
https://www.ncbi.nlm.nih.gov/pubmed/24561827
http://dx.doi.org/10.1016/j.ymeth.2014.01.022
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author Howat, William J.
Wilson, Beverley A.
author_facet Howat, William J.
Wilson, Beverley A.
author_sort Howat, William J.
collection PubMed
description It is impossible to underplay the importance of fixation in histopathology. Whether the scientist is interested in the extraction of information on lipids, proteins, RNA or DNA, fixation is critical to this extraction. This review aims to give a brief overview of the current “state of play” in fixation and focus on the effect fixation, and particularly the effect of the newer brand of “molecular fixatives” have on morphology, histochemistry, immunohistochemistry and RNA/DNA analysis. A methodology incorporating the creation of a fixation tissue microarray for the study of the effect of fixation on histochemistry is detailed.
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spelling pubmed-42408012014-11-25 Tissue fixation and the effect of molecular fixatives on downstream staining procedures Howat, William J. Wilson, Beverley A. Methods Article It is impossible to underplay the importance of fixation in histopathology. Whether the scientist is interested in the extraction of information on lipids, proteins, RNA or DNA, fixation is critical to this extraction. This review aims to give a brief overview of the current “state of play” in fixation and focus on the effect fixation, and particularly the effect of the newer brand of “molecular fixatives” have on morphology, histochemistry, immunohistochemistry and RNA/DNA analysis. A methodology incorporating the creation of a fixation tissue microarray for the study of the effect of fixation on histochemistry is detailed. Academic Press 2014-11 /pmc/articles/PMC4240801/ /pubmed/24561827 http://dx.doi.org/10.1016/j.ymeth.2014.01.022 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Howat, William J.
Wilson, Beverley A.
Tissue fixation and the effect of molecular fixatives on downstream staining procedures
title Tissue fixation and the effect of molecular fixatives on downstream staining procedures
title_full Tissue fixation and the effect of molecular fixatives on downstream staining procedures
title_fullStr Tissue fixation and the effect of molecular fixatives on downstream staining procedures
title_full_unstemmed Tissue fixation and the effect of molecular fixatives on downstream staining procedures
title_short Tissue fixation and the effect of molecular fixatives on downstream staining procedures
title_sort tissue fixation and the effect of molecular fixatives on downstream staining procedures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240801/
https://www.ncbi.nlm.nih.gov/pubmed/24561827
http://dx.doi.org/10.1016/j.ymeth.2014.01.022
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