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A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair. This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context. This...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241339/ https://www.ncbi.nlm.nih.gov/pubmed/25431676 http://dx.doi.org/10.1155/2014/791539 |
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author | Di, Lu-Zhao Couture, Vanessa Leblanc, Élisabeth Alinejad, Yasaman Beaudoin, Jean-François Lecomte, Roger Berthod, François Faucheux, Nathalie Balg, Frédéric Grenier, Guillaume |
author_facet | Di, Lu-Zhao Couture, Vanessa Leblanc, Élisabeth Alinejad, Yasaman Beaudoin, Jean-François Lecomte, Roger Berthod, François Faucheux, Nathalie Balg, Frédéric Grenier, Guillaume |
author_sort | Di, Lu-Zhao |
collection | PubMed |
description | Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair. This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context. This is mainly due to the size of the defects (approximately 1.0 mm) in long bone, which heal rapidly and may thus negatively impact the assessment of the effectiveness of experimental treatments. We developed a longitudinal low dose μCT scan analysis method combined with a new image segmentation and extraction software using Hounsfield unit (HU) scores to quantitatively monitor bone healing in small femoral cortical defects in live mice. We were able to reproducibly quantify bone healing longitudinally over time with three observers. We used high speed intramedullary reaming to prolong healing in order to circumvent the rapid healing typical of small defects. Bone healing prolongation combined with μCT imaging to study small bone defects in live mice thus shows potential as a promising tool for future preclinical research on bone healing. |
format | Online Article Text |
id | pubmed-4241339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42413392014-11-27 A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study Di, Lu-Zhao Couture, Vanessa Leblanc, Élisabeth Alinejad, Yasaman Beaudoin, Jean-François Lecomte, Roger Berthod, François Faucheux, Nathalie Balg, Frédéric Grenier, Guillaume Adv Orthop Research Article Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair. This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context. This is mainly due to the size of the defects (approximately 1.0 mm) in long bone, which heal rapidly and may thus negatively impact the assessment of the effectiveness of experimental treatments. We developed a longitudinal low dose μCT scan analysis method combined with a new image segmentation and extraction software using Hounsfield unit (HU) scores to quantitatively monitor bone healing in small femoral cortical defects in live mice. We were able to reproducibly quantify bone healing longitudinally over time with three observers. We used high speed intramedullary reaming to prolong healing in order to circumvent the rapid healing typical of small defects. Bone healing prolongation combined with μCT imaging to study small bone defects in live mice thus shows potential as a promising tool for future preclinical research on bone healing. Hindawi Publishing Corporation 2014 2014-11-06 /pmc/articles/PMC4241339/ /pubmed/25431676 http://dx.doi.org/10.1155/2014/791539 Text en Copyright © 2014 Lu-Zhao Di et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Di, Lu-Zhao Couture, Vanessa Leblanc, Élisabeth Alinejad, Yasaman Beaudoin, Jean-François Lecomte, Roger Berthod, François Faucheux, Nathalie Balg, Frédéric Grenier, Guillaume A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study |
title | A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study |
title_full | A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study |
title_fullStr | A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study |
title_full_unstemmed | A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study |
title_short | A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study |
title_sort | longitudinal low dose μct analysis of bone healing in mice: a pilot study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241339/ https://www.ncbi.nlm.nih.gov/pubmed/25431676 http://dx.doi.org/10.1155/2014/791539 |
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