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A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study

Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair. This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context. This...

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Autores principales: Di, Lu-Zhao, Couture, Vanessa, Leblanc, Élisabeth, Alinejad, Yasaman, Beaudoin, Jean-François, Lecomte, Roger, Berthod, François, Faucheux, Nathalie, Balg, Frédéric, Grenier, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241339/
https://www.ncbi.nlm.nih.gov/pubmed/25431676
http://dx.doi.org/10.1155/2014/791539
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author Di, Lu-Zhao
Couture, Vanessa
Leblanc, Élisabeth
Alinejad, Yasaman
Beaudoin, Jean-François
Lecomte, Roger
Berthod, François
Faucheux, Nathalie
Balg, Frédéric
Grenier, Guillaume
author_facet Di, Lu-Zhao
Couture, Vanessa
Leblanc, Élisabeth
Alinejad, Yasaman
Beaudoin, Jean-François
Lecomte, Roger
Berthod, François
Faucheux, Nathalie
Balg, Frédéric
Grenier, Guillaume
author_sort Di, Lu-Zhao
collection PubMed
description Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair. This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context. This is mainly due to the size of the defects (approximately 1.0 mm) in long bone, which heal rapidly and may thus negatively impact the assessment of the effectiveness of experimental treatments. We developed a longitudinal low dose μCT scan analysis method combined with a new image segmentation and extraction software using Hounsfield unit (HU) scores to quantitatively monitor bone healing in small femoral cortical defects in live mice. We were able to reproducibly quantify bone healing longitudinally over time with three observers. We used high speed intramedullary reaming to prolong healing in order to circumvent the rapid healing typical of small defects. Bone healing prolongation combined with μCT imaging to study small bone defects in live mice thus shows potential as a promising tool for future preclinical research on bone healing.
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spelling pubmed-42413392014-11-27 A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study Di, Lu-Zhao Couture, Vanessa Leblanc, Élisabeth Alinejad, Yasaman Beaudoin, Jean-François Lecomte, Roger Berthod, François Faucheux, Nathalie Balg, Frédéric Grenier, Guillaume Adv Orthop Research Article Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair. This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context. This is mainly due to the size of the defects (approximately 1.0 mm) in long bone, which heal rapidly and may thus negatively impact the assessment of the effectiveness of experimental treatments. We developed a longitudinal low dose μCT scan analysis method combined with a new image segmentation and extraction software using Hounsfield unit (HU) scores to quantitatively monitor bone healing in small femoral cortical defects in live mice. We were able to reproducibly quantify bone healing longitudinally over time with three observers. We used high speed intramedullary reaming to prolong healing in order to circumvent the rapid healing typical of small defects. Bone healing prolongation combined with μCT imaging to study small bone defects in live mice thus shows potential as a promising tool for future preclinical research on bone healing. Hindawi Publishing Corporation 2014 2014-11-06 /pmc/articles/PMC4241339/ /pubmed/25431676 http://dx.doi.org/10.1155/2014/791539 Text en Copyright © 2014 Lu-Zhao Di et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Di, Lu-Zhao
Couture, Vanessa
Leblanc, Élisabeth
Alinejad, Yasaman
Beaudoin, Jean-François
Lecomte, Roger
Berthod, François
Faucheux, Nathalie
Balg, Frédéric
Grenier, Guillaume
A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
title A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
title_full A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
title_fullStr A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
title_full_unstemmed A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
title_short A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
title_sort longitudinal low dose μct analysis of bone healing in mice: a pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241339/
https://www.ncbi.nlm.nih.gov/pubmed/25431676
http://dx.doi.org/10.1155/2014/791539
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