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MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines
Neuroblastoma is an extra-cranial solid cancer in children. MYCN gene amplification is a prognostic indicator of poor outcome in neuroblastoma. Recent studies have shown that the multiple steps involved in cell migration are dependent on the availability of intracellular calcium (Ca(2+)). Although s...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241534/ https://www.ncbi.nlm.nih.gov/pubmed/25426416 http://dx.doi.org/10.1016/j.fob.2014.10.012 |
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author | Lange, Ingo Koomoa, Dana-Lynn T. |
author_facet | Lange, Ingo Koomoa, Dana-Lynn T. |
author_sort | Lange, Ingo |
collection | PubMed |
description | Neuroblastoma is an extra-cranial solid cancer in children. MYCN gene amplification is a prognostic indicator of poor outcome in neuroblastoma. Recent studies have shown that the multiple steps involved in cell migration are dependent on the availability of intracellular calcium (Ca(2+)). Although significant advances have been made in understanding the role of Ca(2+) during migration, little has been achieved towards understanding its impact on the progression of diseases such as cancer. Interestingly, previous studies showed that cancer cell migration is regulated by TRPM7, a calcium-permeable ion channel. The objective of the current study was to elucidate the mechanism by which MycN promotes NB cell migration and the mechanism regulating TRPM7 expression. The results showed that MycN increased TRPM7 expression, induced TRPM7 channel activity, increased intracellular Ca(2+) signaling, and promoted cell migration in NB cells. The results also showed that inhibition or down-regulation of ornithine decarboxylase (ODC) inhibited TRPM7 expression, a process that was reversed by spermidine. Overall, this study provides evidence that MycN promotes TRPM7 expression and cell migration through a mechanism that involves ODC synthesis of polyamines. |
format | Online Article Text |
id | pubmed-4241534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-42415342014-11-25 MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines Lange, Ingo Koomoa, Dana-Lynn T. FEBS Open Bio Article Neuroblastoma is an extra-cranial solid cancer in children. MYCN gene amplification is a prognostic indicator of poor outcome in neuroblastoma. Recent studies have shown that the multiple steps involved in cell migration are dependent on the availability of intracellular calcium (Ca(2+)). Although significant advances have been made in understanding the role of Ca(2+) during migration, little has been achieved towards understanding its impact on the progression of diseases such as cancer. Interestingly, previous studies showed that cancer cell migration is regulated by TRPM7, a calcium-permeable ion channel. The objective of the current study was to elucidate the mechanism by which MycN promotes NB cell migration and the mechanism regulating TRPM7 expression. The results showed that MycN increased TRPM7 expression, induced TRPM7 channel activity, increased intracellular Ca(2+) signaling, and promoted cell migration in NB cells. The results also showed that inhibition or down-regulation of ornithine decarboxylase (ODC) inhibited TRPM7 expression, a process that was reversed by spermidine. Overall, this study provides evidence that MycN promotes TRPM7 expression and cell migration through a mechanism that involves ODC synthesis of polyamines. Elsevier 2014-10-30 /pmc/articles/PMC4241534/ /pubmed/25426416 http://dx.doi.org/10.1016/j.fob.2014.10.012 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Lange, Ingo Koomoa, Dana-Lynn T. MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines |
title | MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines |
title_full | MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines |
title_fullStr | MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines |
title_full_unstemmed | MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines |
title_short | MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines |
title_sort | mycn promotes trpm7 expression and cell migration in neuroblastoma through a process that involves polyamines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241534/ https://www.ncbi.nlm.nih.gov/pubmed/25426416 http://dx.doi.org/10.1016/j.fob.2014.10.012 |
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