The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes

BACKGROUND: In the Wnt pathway, the secreted frizzled-related protein 2 (SFRP2) is thought to act as one of the several competitive inhibitors of Wnt. However, the precise role of SFRP2 is still poorly understood especially in B lymphocytes. Here, we investigated the function of SFRP2, comparing the...

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Detalles Bibliográficos
Autores principales: Tokuda, Yuichi, Tanaka, Masami, Yagi, Tomohito, Tashiro, Kei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242488/
https://www.ncbi.nlm.nih.gov/pubmed/25370898
http://dx.doi.org/10.1186/1756-0500-7-780
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author Tokuda, Yuichi
Tanaka, Masami
Yagi, Tomohito
Tashiro, Kei
author_facet Tokuda, Yuichi
Tanaka, Masami
Yagi, Tomohito
Tashiro, Kei
author_sort Tokuda, Yuichi
collection PubMed
description BACKGROUND: In the Wnt pathway, the secreted frizzled-related protein 2 (SFRP2) is thought to act as one of the several competitive inhibitors of Wnt. However, the precise role of SFRP2 is still poorly understood especially in B lymphocytes. Here, we investigated the function of SFRP2, comparing the SFRP2 defective as well as normal B lymphocytes in mice. RESULTS: We demonstrated that calcium influx from extracellular to intracellular space in splenic B cells was clearly affected by the defect of SFRP2. In addition, the phosphorylation of phospholipase Cγ2 was observed to be reduced in SFRP2 defective splenic B cells with B cell receptor stimulation. CONCLUSIONS: SFRP2 is suggested to modulate the influx from extracellular calcium in the B cell receptor signaling pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-0500-7-780) contains supplementary material, which is available to authorized users.
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spelling pubmed-42424882014-11-25 The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes Tokuda, Yuichi Tanaka, Masami Yagi, Tomohito Tashiro, Kei BMC Res Notes Research Article BACKGROUND: In the Wnt pathway, the secreted frizzled-related protein 2 (SFRP2) is thought to act as one of the several competitive inhibitors of Wnt. However, the precise role of SFRP2 is still poorly understood especially in B lymphocytes. Here, we investigated the function of SFRP2, comparing the SFRP2 defective as well as normal B lymphocytes in mice. RESULTS: We demonstrated that calcium influx from extracellular to intracellular space in splenic B cells was clearly affected by the defect of SFRP2. In addition, the phosphorylation of phospholipase Cγ2 was observed to be reduced in SFRP2 defective splenic B cells with B cell receptor stimulation. CONCLUSIONS: SFRP2 is suggested to modulate the influx from extracellular calcium in the B cell receptor signaling pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-0500-7-780) contains supplementary material, which is available to authorized users. BioMed Central 2014-11-04 /pmc/articles/PMC4242488/ /pubmed/25370898 http://dx.doi.org/10.1186/1756-0500-7-780 Text en © Tokuda et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Tokuda, Yuichi
Tanaka, Masami
Yagi, Tomohito
Tashiro, Kei
The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
title The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
title_full The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
title_fullStr The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
title_full_unstemmed The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
title_short The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
title_sort defect of sfrp2 modulates an influx of extracellular calcium in b lymphocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242488/
https://www.ncbi.nlm.nih.gov/pubmed/25370898
http://dx.doi.org/10.1186/1756-0500-7-780
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