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Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices
With accelerating rates of obesity and type 2 diabetes world-wide, interest in studying the adipocyte and adipose tissue is increasing. Human adipose derived stem cells - differentiated to adipocytes in vitro - are frequently used as a model system for white adipocytes, as most of their pathways and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242727/ https://www.ncbi.nlm.nih.gov/pubmed/25419971 http://dx.doi.org/10.1371/journal.pone.0113620 |
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author | Brännmark, Cecilia Paul, Alexandra Ribeiro, Diana Magnusson, Björn Brolén, Gabriella Enejder, Annika Forslöw, Anna |
author_facet | Brännmark, Cecilia Paul, Alexandra Ribeiro, Diana Magnusson, Björn Brolén, Gabriella Enejder, Annika Forslöw, Anna |
author_sort | Brännmark, Cecilia |
collection | PubMed |
description | With accelerating rates of obesity and type 2 diabetes world-wide, interest in studying the adipocyte and adipose tissue is increasing. Human adipose derived stem cells - differentiated to adipocytes in vitro - are frequently used as a model system for white adipocytes, as most of their pathways and functions resemble mature adipocytes in vivo. However, these cells are not completely like in vivo mature adipocytes. Hosting the cells in a more physiologically relevant environment compared to conventional two-dimensional cell culturing on plastic surfaces, can produce spatial cues that drive the cells towards a more mature state. We investigated the adipogenesis of adipose derived stem cells on electro spun polycaprolactone matrices and compared functionality to conventional two-dimensional cultures as well as to human primary mature adipocytes. To assess the degree of adipogenesis we measured cellular glucose-uptake and lipolysis and used a range of different methods to evaluate lipid accumulation. We compared the averaged results from a whole population with the single cell characteristics – studied by coherent anti-Stokes Raman scattering microscopy - to gain a comprehensive picture of the cell phenotypes. In adipose derived stem cells differentiated on a polycaprolactone-fiber matrix; an increased sensitivity in insulin-stimulated glucose uptake was detected when cells were grown on either aligned or random matrices. Furthermore, comparing differentiation of adipose derived stem cells on aligned polycaprolactone-fiber matrixes, to those differentiated in two-dimensional cultures showed, an increase in the cellular lipid accumulation, and hormone sensitive lipase content. In conclusion, we propose an adipocyte cell model created by differentiation of adipose derived stem cells on aligned polycaprolactone-fiber matrices which demonstrates increased maturity, compared to 2D cultured cells. |
format | Online Article Text |
id | pubmed-4242727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42427272014-11-26 Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices Brännmark, Cecilia Paul, Alexandra Ribeiro, Diana Magnusson, Björn Brolén, Gabriella Enejder, Annika Forslöw, Anna PLoS One Research Article With accelerating rates of obesity and type 2 diabetes world-wide, interest in studying the adipocyte and adipose tissue is increasing. Human adipose derived stem cells - differentiated to adipocytes in vitro - are frequently used as a model system for white adipocytes, as most of their pathways and functions resemble mature adipocytes in vivo. However, these cells are not completely like in vivo mature adipocytes. Hosting the cells in a more physiologically relevant environment compared to conventional two-dimensional cell culturing on plastic surfaces, can produce spatial cues that drive the cells towards a more mature state. We investigated the adipogenesis of adipose derived stem cells on electro spun polycaprolactone matrices and compared functionality to conventional two-dimensional cultures as well as to human primary mature adipocytes. To assess the degree of adipogenesis we measured cellular glucose-uptake and lipolysis and used a range of different methods to evaluate lipid accumulation. We compared the averaged results from a whole population with the single cell characteristics – studied by coherent anti-Stokes Raman scattering microscopy - to gain a comprehensive picture of the cell phenotypes. In adipose derived stem cells differentiated on a polycaprolactone-fiber matrix; an increased sensitivity in insulin-stimulated glucose uptake was detected when cells were grown on either aligned or random matrices. Furthermore, comparing differentiation of adipose derived stem cells on aligned polycaprolactone-fiber matrixes, to those differentiated in two-dimensional cultures showed, an increase in the cellular lipid accumulation, and hormone sensitive lipase content. In conclusion, we propose an adipocyte cell model created by differentiation of adipose derived stem cells on aligned polycaprolactone-fiber matrices which demonstrates increased maturity, compared to 2D cultured cells. Public Library of Science 2014-11-24 /pmc/articles/PMC4242727/ /pubmed/25419971 http://dx.doi.org/10.1371/journal.pone.0113620 Text en © 2014 Brännmark et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Brännmark, Cecilia Paul, Alexandra Ribeiro, Diana Magnusson, Björn Brolén, Gabriella Enejder, Annika Forslöw, Anna Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices |
title | Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices |
title_full | Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices |
title_fullStr | Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices |
title_full_unstemmed | Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices |
title_short | Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices |
title_sort | increased adipogenesis of human adipose-derived stem cells on polycaprolactone fiber matrices |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242727/ https://www.ncbi.nlm.nih.gov/pubmed/25419971 http://dx.doi.org/10.1371/journal.pone.0113620 |
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