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MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway

Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway....

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Autores principales: Ahmed, Mohammed I., Alam, Majid, Emelianov, Vladimir U., Poterlowicz, Krzysztof, Patel, Ankit, Sharov, Andrey A., Mardaryev, Andrei N., Botchkareva, Natalia V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242830/
https://www.ncbi.nlm.nih.gov/pubmed/25422376
http://dx.doi.org/10.1083/jcb.201404001
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author Ahmed, Mohammed I.
Alam, Majid
Emelianov, Vladimir U.
Poterlowicz, Krzysztof
Patel, Ankit
Sharov, Andrey A.
Mardaryev, Andrei N.
Botchkareva, Natalia V.
author_facet Ahmed, Mohammed I.
Alam, Majid
Emelianov, Vladimir U.
Poterlowicz, Krzysztof
Patel, Ankit
Sharov, Andrey A.
Mardaryev, Andrei N.
Botchkareva, Natalia V.
author_sort Ahmed, Mohammed I.
collection PubMed
description Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway. miR-214 exhibits differential expression patterns in the skin epithelium, and its inducible overexpression in keratinocytes inhibited proliferation, which resulted in formation of fewer HFs with decreased hair bulb size and thinner hair production. The inhibitory effects of miR-214 on HF development and cycling were associated with altered activities of multiple signaling pathways, including decreased expression of key Wnt signaling mediators β-catenin and Lef-1, and were rescued by treatment with pharmacological Wnt activators. Finally, we identify β-catenin as one of the conserved miR-214 targets in keratinocytes. These data provide an important foundation for further analyses of miR-214 as a key regulator of Wnt pathway activity and stem cell functions during normal tissue homeostasis, regeneration, and aging.
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spelling pubmed-42428302015-05-24 MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway Ahmed, Mohammed I. Alam, Majid Emelianov, Vladimir U. Poterlowicz, Krzysztof Patel, Ankit Sharov, Andrey A. Mardaryev, Andrei N. Botchkareva, Natalia V. J Cell Biol Research Articles Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway. miR-214 exhibits differential expression patterns in the skin epithelium, and its inducible overexpression in keratinocytes inhibited proliferation, which resulted in formation of fewer HFs with decreased hair bulb size and thinner hair production. The inhibitory effects of miR-214 on HF development and cycling were associated with altered activities of multiple signaling pathways, including decreased expression of key Wnt signaling mediators β-catenin and Lef-1, and were rescued by treatment with pharmacological Wnt activators. Finally, we identify β-catenin as one of the conserved miR-214 targets in keratinocytes. These data provide an important foundation for further analyses of miR-214 as a key regulator of Wnt pathway activity and stem cell functions during normal tissue homeostasis, regeneration, and aging. The Rockefeller University Press 2014-11-24 /pmc/articles/PMC4242830/ /pubmed/25422376 http://dx.doi.org/10.1083/jcb.201404001 Text en © 2014 Ahmed et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Ahmed, Mohammed I.
Alam, Majid
Emelianov, Vladimir U.
Poterlowicz, Krzysztof
Patel, Ankit
Sharov, Andrey A.
Mardaryev, Andrei N.
Botchkareva, Natalia V.
MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway
title MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway
title_full MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway
title_fullStr MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway
title_full_unstemmed MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway
title_short MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway
title_sort microrna-214 controls skin and hair follicle development by modulating the activity of the wnt pathway
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242830/
https://www.ncbi.nlm.nih.gov/pubmed/25422376
http://dx.doi.org/10.1083/jcb.201404001
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