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Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections
Healthcare-associated infections (HAI) are a huge public health concern, particularly when the etiological agents are multidrug resistant. The ability of bacteria to develop biofilm is a helpful skill, both to persist within hospital units and to increase antibiotic resistance. Although the links be...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243437/ https://www.ncbi.nlm.nih.gov/pubmed/25438020 http://dx.doi.org/10.3390/pathogens3030720 |
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author | Bandeira, Maria Almeida Carvalho, Patricia Duarte, Aida Jordao, Luisa |
author_facet | Bandeira, Maria Almeida Carvalho, Patricia Duarte, Aida Jordao, Luisa |
author_sort | Bandeira, Maria |
collection | PubMed |
description | Healthcare-associated infections (HAI) are a huge public health concern, particularly when the etiological agents are multidrug resistant. The ability of bacteria to develop biofilm is a helpful skill, both to persist within hospital units and to increase antibiotic resistance. Although the links between antibiotic resistance, biofilms assembly and HAI are consensual, little is known about biofilms. Here, electron microscopy was adopted as a tool to investigate biofilm structures associated with increased antibiotic resistance. The K. pneumoniae strains investigated are able to assemble biofilms, albeit with different kinetics. The biofilm structure and the relative area fractions of bacteria and extracellular matrix depend on the particular strain, as well as the minimal inhibitory concentration (MIC) for the antibiotics. Increased values were found for bacteria organized in biofilms when compared to the respective planktonic forms, except for isolates Kp45 and Kp2948, the MIC values for which remained unchanged for fosfomycin. Altogether, these results showed that the emergence of antimicrobial resistance among bacteria responsible for HAI is a multifactorial phenomenon dependent on antibiotics and on bacteria/biofilm features. |
format | Online Article Text |
id | pubmed-4243437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42434372014-11-25 Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections Bandeira, Maria Almeida Carvalho, Patricia Duarte, Aida Jordao, Luisa Pathogens Article Healthcare-associated infections (HAI) are a huge public health concern, particularly when the etiological agents are multidrug resistant. The ability of bacteria to develop biofilm is a helpful skill, both to persist within hospital units and to increase antibiotic resistance. Although the links between antibiotic resistance, biofilms assembly and HAI are consensual, little is known about biofilms. Here, electron microscopy was adopted as a tool to investigate biofilm structures associated with increased antibiotic resistance. The K. pneumoniae strains investigated are able to assemble biofilms, albeit with different kinetics. The biofilm structure and the relative area fractions of bacteria and extracellular matrix depend on the particular strain, as well as the minimal inhibitory concentration (MIC) for the antibiotics. Increased values were found for bacteria organized in biofilms when compared to the respective planktonic forms, except for isolates Kp45 and Kp2948, the MIC values for which remained unchanged for fosfomycin. Altogether, these results showed that the emergence of antimicrobial resistance among bacteria responsible for HAI is a multifactorial phenomenon dependent on antibiotics and on bacteria/biofilm features. MDPI 2014-08-19 /pmc/articles/PMC4243437/ /pubmed/25438020 http://dx.doi.org/10.3390/pathogens3030720 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Bandeira, Maria Almeida Carvalho, Patricia Duarte, Aida Jordao, Luisa Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections |
title | Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections |
title_full | Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections |
title_fullStr | Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections |
title_full_unstemmed | Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections |
title_short | Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections |
title_sort | exploring dangerous connections between klebsiella pneumoniae biofilms and healthcare-associated infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243437/ https://www.ncbi.nlm.nih.gov/pubmed/25438020 http://dx.doi.org/10.3390/pathogens3030720 |
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