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In-house development of an optimized synthetic module for routine [(11)C]acetate production
[(11)C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[(18)F]fluoro-d-glucose in a number of cancers. Here, the optimized automatic synthesis of [(11)C]acetate using an in-house-developed module under different conditions has bee...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243701/ https://www.ncbi.nlm.nih.gov/pubmed/25244351 http://dx.doi.org/10.1097/MNM.0000000000000213 |
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author | Jang, Hwa Youn Kwon, Seong Young Pyo, Ayoung Hur, Min Goo Kim, Sang Wook Park, Jeong-Hoon Kim, Hee-Jung Yang, Seung Dae Lee, Sunwoo Kim, Dong-Yeon Min, Jung-Joon |
author_facet | Jang, Hwa Youn Kwon, Seong Young Pyo, Ayoung Hur, Min Goo Kim, Sang Wook Park, Jeong-Hoon Kim, Hee-Jung Yang, Seung Dae Lee, Sunwoo Kim, Dong-Yeon Min, Jung-Joon |
author_sort | Jang, Hwa Youn |
collection | PubMed |
description | [(11)C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[(18)F]fluoro-d-glucose in a number of cancers. Here, the optimized automatic synthesis of [(11)C]acetate using an in-house-developed module under different conditions has been reported for routine production. [(11)C]CO(2) was produced in a 16.4 MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction cartridges were used, connected in series. High-performance liquid chromatography and gas chromatography were used to measure radiochemical and chemical purity. The Limulus amebocyte lysate test and the fluid thioglycollate medium test were performed for quality control of [(11)C]acetate. The total reaction time of [(11)C]acetate was within 15 min, and the overall decay-corrected radiochemical yield was 84.33±8.85%. Radiochemical purity was greater than 98% when evaluated on an analytical high-performance liquid chromatography system. No endotoxins or anaerobic bacteria were seen on quality control checks. Optimized production of [(11)C]acetate was achieved by the in-house module. Radiochemical and biological properties of the [(11)C]acetate produced were appropriate for clinical PET study. |
format | Online Article Text |
id | pubmed-4243701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-42437012014-11-26 In-house development of an optimized synthetic module for routine [(11)C]acetate production Jang, Hwa Youn Kwon, Seong Young Pyo, Ayoung Hur, Min Goo Kim, Sang Wook Park, Jeong-Hoon Kim, Hee-Jung Yang, Seung Dae Lee, Sunwoo Kim, Dong-Yeon Min, Jung-Joon Nucl Med Commun Technical Notes [(11)C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[(18)F]fluoro-d-glucose in a number of cancers. Here, the optimized automatic synthesis of [(11)C]acetate using an in-house-developed module under different conditions has been reported for routine production. [(11)C]CO(2) was produced in a 16.4 MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction cartridges were used, connected in series. High-performance liquid chromatography and gas chromatography were used to measure radiochemical and chemical purity. The Limulus amebocyte lysate test and the fluid thioglycollate medium test were performed for quality control of [(11)C]acetate. The total reaction time of [(11)C]acetate was within 15 min, and the overall decay-corrected radiochemical yield was 84.33±8.85%. Radiochemical purity was greater than 98% when evaluated on an analytical high-performance liquid chromatography system. No endotoxins or anaerobic bacteria were seen on quality control checks. Optimized production of [(11)C]acetate was achieved by the in-house module. Radiochemical and biological properties of the [(11)C]acetate produced were appropriate for clinical PET study. Lippincott Williams & Wilkins 2015-01 2014-11-27 /pmc/articles/PMC4243701/ /pubmed/25244351 http://dx.doi.org/10.1097/MNM.0000000000000213 Text en © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivitives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially. http://creativecommons.org/licenses/by-nc-nd/3.0. |
spellingShingle | Technical Notes Jang, Hwa Youn Kwon, Seong Young Pyo, Ayoung Hur, Min Goo Kim, Sang Wook Park, Jeong-Hoon Kim, Hee-Jung Yang, Seung Dae Lee, Sunwoo Kim, Dong-Yeon Min, Jung-Joon In-house development of an optimized synthetic module for routine [(11)C]acetate production |
title | In-house development of an optimized synthetic module for routine [(11)C]acetate production |
title_full | In-house development of an optimized synthetic module for routine [(11)C]acetate production |
title_fullStr | In-house development of an optimized synthetic module for routine [(11)C]acetate production |
title_full_unstemmed | In-house development of an optimized synthetic module for routine [(11)C]acetate production |
title_short | In-house development of an optimized synthetic module for routine [(11)C]acetate production |
title_sort | in-house development of an optimized synthetic module for routine [(11)c]acetate production |
topic | Technical Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243701/ https://www.ncbi.nlm.nih.gov/pubmed/25244351 http://dx.doi.org/10.1097/MNM.0000000000000213 |
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