Cargando…

In-house development of an optimized synthetic module for routine [(11)C]acetate production

[(11)C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[(18)F]fluoro-d-glucose in a number of cancers. Here, the optimized automatic synthesis of [(11)C]acetate using an in-house-developed module under different conditions has bee...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, Hwa Youn, Kwon, Seong Young, Pyo, Ayoung, Hur, Min Goo, Kim, Sang Wook, Park, Jeong-Hoon, Kim, Hee-Jung, Yang, Seung Dae, Lee, Sunwoo, Kim, Dong-Yeon, Min, Jung-Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243701/
https://www.ncbi.nlm.nih.gov/pubmed/25244351
http://dx.doi.org/10.1097/MNM.0000000000000213
_version_ 1782346131114557440
author Jang, Hwa Youn
Kwon, Seong Young
Pyo, Ayoung
Hur, Min Goo
Kim, Sang Wook
Park, Jeong-Hoon
Kim, Hee-Jung
Yang, Seung Dae
Lee, Sunwoo
Kim, Dong-Yeon
Min, Jung-Joon
author_facet Jang, Hwa Youn
Kwon, Seong Young
Pyo, Ayoung
Hur, Min Goo
Kim, Sang Wook
Park, Jeong-Hoon
Kim, Hee-Jung
Yang, Seung Dae
Lee, Sunwoo
Kim, Dong-Yeon
Min, Jung-Joon
author_sort Jang, Hwa Youn
collection PubMed
description [(11)C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[(18)F]fluoro-d-glucose in a number of cancers. Here, the optimized automatic synthesis of [(11)C]acetate using an in-house-developed module under different conditions has been reported for routine production. [(11)C]CO(2) was produced in a 16.4 MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction cartridges were used, connected in series. High-performance liquid chromatography and gas chromatography were used to measure radiochemical and chemical purity. The Limulus amebocyte lysate test and the fluid thioglycollate medium test were performed for quality control of [(11)C]acetate. The total reaction time of [(11)C]acetate was within 15 min, and the overall decay-corrected radiochemical yield was 84.33±8.85%. Radiochemical purity was greater than 98% when evaluated on an analytical high-performance liquid chromatography system. No endotoxins or anaerobic bacteria were seen on quality control checks. Optimized production of [(11)C]acetate was achieved by the in-house module. Radiochemical and biological properties of the [(11)C]acetate produced were appropriate for clinical PET study.
format Online
Article
Text
id pubmed-4243701
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-42437012014-11-26 In-house development of an optimized synthetic module for routine [(11)C]acetate production Jang, Hwa Youn Kwon, Seong Young Pyo, Ayoung Hur, Min Goo Kim, Sang Wook Park, Jeong-Hoon Kim, Hee-Jung Yang, Seung Dae Lee, Sunwoo Kim, Dong-Yeon Min, Jung-Joon Nucl Med Commun Technical Notes [(11)C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[(18)F]fluoro-d-glucose in a number of cancers. Here, the optimized automatic synthesis of [(11)C]acetate using an in-house-developed module under different conditions has been reported for routine production. [(11)C]CO(2) was produced in a 16.4 MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction cartridges were used, connected in series. High-performance liquid chromatography and gas chromatography were used to measure radiochemical and chemical purity. The Limulus amebocyte lysate test and the fluid thioglycollate medium test were performed for quality control of [(11)C]acetate. The total reaction time of [(11)C]acetate was within 15 min, and the overall decay-corrected radiochemical yield was 84.33±8.85%. Radiochemical purity was greater than 98% when evaluated on an analytical high-performance liquid chromatography system. No endotoxins or anaerobic bacteria were seen on quality control checks. Optimized production of [(11)C]acetate was achieved by the in-house module. Radiochemical and biological properties of the [(11)C]acetate produced were appropriate for clinical PET study. Lippincott Williams & Wilkins 2015-01 2014-11-27 /pmc/articles/PMC4243701/ /pubmed/25244351 http://dx.doi.org/10.1097/MNM.0000000000000213 Text en © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivitives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially. http://creativecommons.org/licenses/by-nc-nd/3.0.
spellingShingle Technical Notes
Jang, Hwa Youn
Kwon, Seong Young
Pyo, Ayoung
Hur, Min Goo
Kim, Sang Wook
Park, Jeong-Hoon
Kim, Hee-Jung
Yang, Seung Dae
Lee, Sunwoo
Kim, Dong-Yeon
Min, Jung-Joon
In-house development of an optimized synthetic module for routine [(11)C]acetate production
title In-house development of an optimized synthetic module for routine [(11)C]acetate production
title_full In-house development of an optimized synthetic module for routine [(11)C]acetate production
title_fullStr In-house development of an optimized synthetic module for routine [(11)C]acetate production
title_full_unstemmed In-house development of an optimized synthetic module for routine [(11)C]acetate production
title_short In-house development of an optimized synthetic module for routine [(11)C]acetate production
title_sort in-house development of an optimized synthetic module for routine [(11)c]acetate production
topic Technical Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243701/
https://www.ncbi.nlm.nih.gov/pubmed/25244351
http://dx.doi.org/10.1097/MNM.0000000000000213
work_keys_str_mv AT janghwayoun inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT kwonseongyoung inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT pyoayoung inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT hurmingoo inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT kimsangwook inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT parkjeonghoon inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT kimheejung inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT yangseungdae inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT leesunwoo inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT kimdongyeon inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction
AT minjungjoon inhousedevelopmentofanoptimizedsyntheticmoduleforroutine11cacetateproduction