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In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals
OBJECTIVE: The purpose of this study was to evaluate the efficacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. MATERIAL AND METHODS: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups accordi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade de Odontologia de Bauru da Universidade de São
Paulo
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243747/ https://www.ncbi.nlm.nih.gov/pubmed/21552710 http://dx.doi.org/10.1590/S1678-77572011000200005 |
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author | MAEKAWA, Lilian Eiko VALERA, Marcia Carneiro de OLIVEIRA, Luciane Dias CARVALHO, Cláudio Antonio Talge KOGA-ITO, Cristiane Yumi JORGE, Antonio Olavo Cardoso |
author_facet | MAEKAWA, Lilian Eiko VALERA, Marcia Carneiro de OLIVEIRA, Luciane Dias CARVALHO, Cláudio Antonio Talge KOGA-ITO, Cristiane Yumi JORGE, Antonio Olavo Cardoso |
author_sort | MAEKAWA, Lilian Eiko |
collection | PubMed |
description | OBJECTIVE: The purpose of this study was to evaluate the efficacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. MATERIAL AND METHODS: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used: G1) 2.5% sodium hypochlorite (NaOCl); G2) 2% chlorhexidine gel (CLX); G3) pyrogen-free solution. After, these groups were subdivided according to the intracanal medication (ICM): A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2% CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections: a) antimicrobial activity; b) quantification of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn’s tests at 5% significance level. RESULTS: The 2.5% NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline. CONCLUSIONS: It was concluded that 2.5% NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used. |
format | Online Article Text |
id | pubmed-4243747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Faculdade de Odontologia de Bauru da Universidade de São
Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-42437472014-11-26 In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals MAEKAWA, Lilian Eiko VALERA, Marcia Carneiro de OLIVEIRA, Luciane Dias CARVALHO, Cláudio Antonio Talge KOGA-ITO, Cristiane Yumi JORGE, Antonio Olavo Cardoso J Appl Oral Sci Original Articles OBJECTIVE: The purpose of this study was to evaluate the efficacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. MATERIAL AND METHODS: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used: G1) 2.5% sodium hypochlorite (NaOCl); G2) 2% chlorhexidine gel (CLX); G3) pyrogen-free solution. After, these groups were subdivided according to the intracanal medication (ICM): A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2% CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections: a) antimicrobial activity; b) quantification of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn’s tests at 5% significance level. RESULTS: The 2.5% NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline. CONCLUSIONS: It was concluded that 2.5% NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2011 /pmc/articles/PMC4243747/ /pubmed/21552710 http://dx.doi.org/10.1590/S1678-77572011000200005 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles MAEKAWA, Lilian Eiko VALERA, Marcia Carneiro de OLIVEIRA, Luciane Dias CARVALHO, Cláudio Antonio Talge KOGA-ITO, Cristiane Yumi JORGE, Antonio Olavo Cardoso In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
title | In vitro evaluation of the action of irrigating
solutions associated with intracanal medications on Escherichia coli
and its endotoxin in root canals |
title_full | In vitro evaluation of the action of irrigating
solutions associated with intracanal medications on Escherichia coli
and its endotoxin in root canals |
title_fullStr | In vitro evaluation of the action of irrigating
solutions associated with intracanal medications on Escherichia coli
and its endotoxin in root canals |
title_full_unstemmed | In vitro evaluation of the action of irrigating
solutions associated with intracanal medications on Escherichia coli
and its endotoxin in root canals |
title_short | In vitro evaluation of the action of irrigating
solutions associated with intracanal medications on Escherichia coli
and its endotoxin in root canals |
title_sort | in vitro evaluation of the action of irrigating
solutions associated with intracanal medications on escherichia coli
and its endotoxin in root canals |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243747/ https://www.ncbi.nlm.nih.gov/pubmed/21552710 http://dx.doi.org/10.1590/S1678-77572011000200005 |
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