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LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2
Recent studies have revealed that long non-coding RNAs participate in all steps of cancer initiation and progression by regulating protein-coding genes at the epigenetic, transcriptional, and post-transcriptional levels. Long non-coding RNAs are in turn regulated by other genes, forming a complex re...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244030/ https://www.ncbi.nlm.nih.gov/pubmed/25422887 http://dx.doi.org/10.1371/journal.pone.0110674 |
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author | Gong, Zhaojian Zhang, Shanshan Zeng, Zhaoyang Wu, Hanjiang Yang, Qian Xiong, Fang Shi, Lei Yang, Jianbo Zhang, Wenling Zhou, Yanhong Zeng, Yong Li, Xiayu Xiang, Bo Peng, Shuping Zhou, Ming Li, Xiaoling Tan, Ming Li, Yong Xiong, Wei Li, Guiyuan |
author_facet | Gong, Zhaojian Zhang, Shanshan Zeng, Zhaoyang Wu, Hanjiang Yang, Qian Xiong, Fang Shi, Lei Yang, Jianbo Zhang, Wenling Zhou, Yanhong Zeng, Yong Li, Xiayu Xiang, Bo Peng, Shuping Zhou, Ming Li, Xiaoling Tan, Ming Li, Yong Xiong, Wei Li, Guiyuan |
author_sort | Gong, Zhaojian |
collection | PubMed |
description | Recent studies have revealed that long non-coding RNAs participate in all steps of cancer initiation and progression by regulating protein-coding genes at the epigenetic, transcriptional, and post-transcriptional levels. Long non-coding RNAs are in turn regulated by other genes, forming a complex regulatory network. The regulation networks between the p53 tumor suppressor and these RNAs in nasopharyngeal carcinoma remains unclear. The aims of this study were to investigate the regulatory roles of the TP53 gene in regulating long non-coding RNA expression profiles and to study the function of a TP53-regulated long non-coding RNA (LOC401317) in the nasopharyngeal carcinoma cell line HNE2. Long non-coding RNA expression profiling indicated that 133 long non-coding RNAs were upregulated in the human NPC cell line HNE2 cells following TP53 overexpression, while 1057 were downregulated. Among these aberrantly expressed long non-coding RNAs, LOC401317 was the most significantly upregulated one. Further studies indicated that LOC401317 is directly regulated by p53 and that ectopic expression of LOC401317 inhibits HNE2 cell proliferation in vitro and in vivo by inducing cell cycle arrest and apoptosis. LOC401317 inhibited cell cycle progression by increasing p21 expression and decreasing cyclin D1 and cyclin E1 expression and promoted apoptosis through the induction of poly(ADP-ribose) polymerase and caspase-3 cleavage. Collectively, these results suggest that LOC401317 is directly regulated by p53 and exerts antitumor effects in HNE2 nasopharyngeal carcinoma cells. |
format | Online Article Text |
id | pubmed-4244030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42440302014-12-05 LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 Gong, Zhaojian Zhang, Shanshan Zeng, Zhaoyang Wu, Hanjiang Yang, Qian Xiong, Fang Shi, Lei Yang, Jianbo Zhang, Wenling Zhou, Yanhong Zeng, Yong Li, Xiayu Xiang, Bo Peng, Shuping Zhou, Ming Li, Xiaoling Tan, Ming Li, Yong Xiong, Wei Li, Guiyuan PLoS One Research Article Recent studies have revealed that long non-coding RNAs participate in all steps of cancer initiation and progression by regulating protein-coding genes at the epigenetic, transcriptional, and post-transcriptional levels. Long non-coding RNAs are in turn regulated by other genes, forming a complex regulatory network. The regulation networks between the p53 tumor suppressor and these RNAs in nasopharyngeal carcinoma remains unclear. The aims of this study were to investigate the regulatory roles of the TP53 gene in regulating long non-coding RNA expression profiles and to study the function of a TP53-regulated long non-coding RNA (LOC401317) in the nasopharyngeal carcinoma cell line HNE2. Long non-coding RNA expression profiling indicated that 133 long non-coding RNAs were upregulated in the human NPC cell line HNE2 cells following TP53 overexpression, while 1057 were downregulated. Among these aberrantly expressed long non-coding RNAs, LOC401317 was the most significantly upregulated one. Further studies indicated that LOC401317 is directly regulated by p53 and that ectopic expression of LOC401317 inhibits HNE2 cell proliferation in vitro and in vivo by inducing cell cycle arrest and apoptosis. LOC401317 inhibited cell cycle progression by increasing p21 expression and decreasing cyclin D1 and cyclin E1 expression and promoted apoptosis through the induction of poly(ADP-ribose) polymerase and caspase-3 cleavage. Collectively, these results suggest that LOC401317 is directly regulated by p53 and exerts antitumor effects in HNE2 nasopharyngeal carcinoma cells. Public Library of Science 2014-11-25 /pmc/articles/PMC4244030/ /pubmed/25422887 http://dx.doi.org/10.1371/journal.pone.0110674 Text en © 2014 Gong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Gong, Zhaojian Zhang, Shanshan Zeng, Zhaoyang Wu, Hanjiang Yang, Qian Xiong, Fang Shi, Lei Yang, Jianbo Zhang, Wenling Zhou, Yanhong Zeng, Yong Li, Xiayu Xiang, Bo Peng, Shuping Zhou, Ming Li, Xiaoling Tan, Ming Li, Yong Xiong, Wei Li, Guiyuan LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 |
title | LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 |
title_full | LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 |
title_fullStr | LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 |
title_full_unstemmed | LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 |
title_short | LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2 |
title_sort | loc401317, a p53-regulated long non-coding rna, inhibits cell proliferation and induces apoptosis in the nasopharyngeal carcinoma cell line hne2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244030/ https://www.ncbi.nlm.nih.gov/pubmed/25422887 http://dx.doi.org/10.1371/journal.pone.0110674 |
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