Cargando…

Exploiting Issatchenkia orientalis SD108 for succinic acid production

BACKGROUND: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Han, Shao, Zengyi, Jiang, Yu, Dole, Sudhanshu, Zhao, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244060/
https://www.ncbi.nlm.nih.gov/pubmed/25159171
http://dx.doi.org/10.1186/s12934-014-0121-4
_version_ 1782346179019800576
author Xiao, Han
Shao, Zengyi
Jiang, Yu
Dole, Sudhanshu
Zhao, Huimin
author_facet Xiao, Han
Shao, Zengyi
Jiang, Yu
Dole, Sudhanshu
Zhao, Huimin
author_sort Xiao, Han
collection PubMed
description BACKGROUND: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable. RESULTS: Here we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 and Classic Distiller’s Turbo yeast. In order to explore SD108 as a potential platform organism for organic acid production, we determined its draft genome sequence and use the sequencing information to guide pathway design. As proof of concept, an engineered four-gene expression cassette related to the reductive TCA cycle was assembled and integrated into the genome of a uracil auxotroph of SD108. The resulting strain was able to produce succinic acid with a titer of 11.63 g/L, yield of 0.12 g/g, and productivity of 0.11 g/L · h in batch cultures using shake flasks. CONCLUSIONS: The high tolerance of I. orientalis SD108 towards multiple important organic acids makes it a highly attractive organism as a platform host for producing this group of compounds as it will reduce production cost, facilitate downstream processing, and serve as a host for construction of production strains with both pH and specific anion tolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-014-0121-4) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4244060
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42440602014-11-26 Exploiting Issatchenkia orientalis SD108 for succinic acid production Xiao, Han Shao, Zengyi Jiang, Yu Dole, Sudhanshu Zhao, Huimin Microb Cell Fact Research BACKGROUND: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable. RESULTS: Here we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 and Classic Distiller’s Turbo yeast. In order to explore SD108 as a potential platform organism for organic acid production, we determined its draft genome sequence and use the sequencing information to guide pathway design. As proof of concept, an engineered four-gene expression cassette related to the reductive TCA cycle was assembled and integrated into the genome of a uracil auxotroph of SD108. The resulting strain was able to produce succinic acid with a titer of 11.63 g/L, yield of 0.12 g/g, and productivity of 0.11 g/L · h in batch cultures using shake flasks. CONCLUSIONS: The high tolerance of I. orientalis SD108 towards multiple important organic acids makes it a highly attractive organism as a platform host for producing this group of compounds as it will reduce production cost, facilitate downstream processing, and serve as a host for construction of production strains with both pH and specific anion tolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-014-0121-4) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-27 /pmc/articles/PMC4244060/ /pubmed/25159171 http://dx.doi.org/10.1186/s12934-014-0121-4 Text en © Xiao et al.; licensee BioMed Central 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xiao, Han
Shao, Zengyi
Jiang, Yu
Dole, Sudhanshu
Zhao, Huimin
Exploiting Issatchenkia orientalis SD108 for succinic acid production
title Exploiting Issatchenkia orientalis SD108 for succinic acid production
title_full Exploiting Issatchenkia orientalis SD108 for succinic acid production
title_fullStr Exploiting Issatchenkia orientalis SD108 for succinic acid production
title_full_unstemmed Exploiting Issatchenkia orientalis SD108 for succinic acid production
title_short Exploiting Issatchenkia orientalis SD108 for succinic acid production
title_sort exploiting issatchenkia orientalis sd108 for succinic acid production
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244060/
https://www.ncbi.nlm.nih.gov/pubmed/25159171
http://dx.doi.org/10.1186/s12934-014-0121-4
work_keys_str_mv AT xiaohan exploitingissatchenkiaorientalissd108forsuccinicacidproduction
AT shaozengyi exploitingissatchenkiaorientalissd108forsuccinicacidproduction
AT jiangyu exploitingissatchenkiaorientalissd108forsuccinicacidproduction
AT dolesudhanshu exploitingissatchenkiaorientalissd108forsuccinicacidproduction
AT zhaohuimin exploitingissatchenkiaorientalissd108forsuccinicacidproduction