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Exploiting Issatchenkia orientalis SD108 for succinic acid production
BACKGROUND: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentatio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244060/ https://www.ncbi.nlm.nih.gov/pubmed/25159171 http://dx.doi.org/10.1186/s12934-014-0121-4 |
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author | Xiao, Han Shao, Zengyi Jiang, Yu Dole, Sudhanshu Zhao, Huimin |
author_facet | Xiao, Han Shao, Zengyi Jiang, Yu Dole, Sudhanshu Zhao, Huimin |
author_sort | Xiao, Han |
collection | PubMed |
description | BACKGROUND: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable. RESULTS: Here we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 and Classic Distiller’s Turbo yeast. In order to explore SD108 as a potential platform organism for organic acid production, we determined its draft genome sequence and use the sequencing information to guide pathway design. As proof of concept, an engineered four-gene expression cassette related to the reductive TCA cycle was assembled and integrated into the genome of a uracil auxotroph of SD108. The resulting strain was able to produce succinic acid with a titer of 11.63 g/L, yield of 0.12 g/g, and productivity of 0.11 g/L · h in batch cultures using shake flasks. CONCLUSIONS: The high tolerance of I. orientalis SD108 towards multiple important organic acids makes it a highly attractive organism as a platform host for producing this group of compounds as it will reduce production cost, facilitate downstream processing, and serve as a host for construction of production strains with both pH and specific anion tolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-014-0121-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4244060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42440602014-11-26 Exploiting Issatchenkia orientalis SD108 for succinic acid production Xiao, Han Shao, Zengyi Jiang, Yu Dole, Sudhanshu Zhao, Huimin Microb Cell Fact Research BACKGROUND: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable. RESULTS: Here we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 and Classic Distiller’s Turbo yeast. In order to explore SD108 as a potential platform organism for organic acid production, we determined its draft genome sequence and use the sequencing information to guide pathway design. As proof of concept, an engineered four-gene expression cassette related to the reductive TCA cycle was assembled and integrated into the genome of a uracil auxotroph of SD108. The resulting strain was able to produce succinic acid with a titer of 11.63 g/L, yield of 0.12 g/g, and productivity of 0.11 g/L · h in batch cultures using shake flasks. CONCLUSIONS: The high tolerance of I. orientalis SD108 towards multiple important organic acids makes it a highly attractive organism as a platform host for producing this group of compounds as it will reduce production cost, facilitate downstream processing, and serve as a host for construction of production strains with both pH and specific anion tolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-014-0121-4) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-27 /pmc/articles/PMC4244060/ /pubmed/25159171 http://dx.doi.org/10.1186/s12934-014-0121-4 Text en © Xiao et al.; licensee BioMed Central 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Xiao, Han Shao, Zengyi Jiang, Yu Dole, Sudhanshu Zhao, Huimin Exploiting Issatchenkia orientalis SD108 for succinic acid production |
title | Exploiting Issatchenkia orientalis SD108 for succinic acid production |
title_full | Exploiting Issatchenkia orientalis SD108 for succinic acid production |
title_fullStr | Exploiting Issatchenkia orientalis SD108 for succinic acid production |
title_full_unstemmed | Exploiting Issatchenkia orientalis SD108 for succinic acid production |
title_short | Exploiting Issatchenkia orientalis SD108 for succinic acid production |
title_sort | exploiting issatchenkia orientalis sd108 for succinic acid production |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244060/ https://www.ncbi.nlm.nih.gov/pubmed/25159171 http://dx.doi.org/10.1186/s12934-014-0121-4 |
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