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The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L

Capparis spinosa is one of the most important eremophytes among the medicinal plants, and continued destruction of these plants poses a major threat to species survival. The development of methods to extract compounds, especially those of medicinal value, without harvesting the whole plant is an iss...

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Autores principales: Yin, Yongtai, He, Yuchi, Liu, Wei, Gan, Lu, Fu, Chunhua, Jia, Haibo, Li, Maoteng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244143/
https://www.ncbi.nlm.nih.gov/pubmed/25422894
http://dx.doi.org/10.1371/journal.pone.0113668
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author Yin, Yongtai
He, Yuchi
Liu, Wei
Gan, Lu
Fu, Chunhua
Jia, Haibo
Li, Maoteng
author_facet Yin, Yongtai
He, Yuchi
Liu, Wei
Gan, Lu
Fu, Chunhua
Jia, Haibo
Li, Maoteng
author_sort Yin, Yongtai
collection PubMed
description Capparis spinosa is one of the most important eremophytes among the medicinal plants, and continued destruction of these plants poses a major threat to species survival. The development of methods to extract compounds, especially those of medicinal value, without harvesting the whole plant is an issue of considerable socioeconomic importance. On the basis of an established system for culture of suspension cells and callus in vitro, Gas Chromatograph-Mass Spectrometer (GC-MS) was used for the volatile oil composition analyzing in seed, fruit, suspension cells and callus. Fatty acids were the major component, and the highest content of alkanes was detected in seed, with <1.0% in suspension cells and callus. Esters, olefins and heterocyclic compounds were significantly higher in fruit than in the other materials. The content of acid esters in the suspension cells and callus was significantly higher than in seed and fruit. This indicated that the suspension cells and callus could be helpful for increasing the value of volatile oil and replacing seeds and fruit partially as a source of some compounds of the volatile oil and may also produce some new medical compounds. The above results give valuable information for sustainable use of C. spinosa and provide a foundation for use of the C. spinosa suspension cells and callus as an ongoing medical resource.
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spelling pubmed-42441432014-12-05 The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L Yin, Yongtai He, Yuchi Liu, Wei Gan, Lu Fu, Chunhua Jia, Haibo Li, Maoteng PLoS One Research Article Capparis spinosa is one of the most important eremophytes among the medicinal plants, and continued destruction of these plants poses a major threat to species survival. The development of methods to extract compounds, especially those of medicinal value, without harvesting the whole plant is an issue of considerable socioeconomic importance. On the basis of an established system for culture of suspension cells and callus in vitro, Gas Chromatograph-Mass Spectrometer (GC-MS) was used for the volatile oil composition analyzing in seed, fruit, suspension cells and callus. Fatty acids were the major component, and the highest content of alkanes was detected in seed, with <1.0% in suspension cells and callus. Esters, olefins and heterocyclic compounds were significantly higher in fruit than in the other materials. The content of acid esters in the suspension cells and callus was significantly higher than in seed and fruit. This indicated that the suspension cells and callus could be helpful for increasing the value of volatile oil and replacing seeds and fruit partially as a source of some compounds of the volatile oil and may also produce some new medical compounds. The above results give valuable information for sustainable use of C. spinosa and provide a foundation for use of the C. spinosa suspension cells and callus as an ongoing medical resource. Public Library of Science 2014-11-25 /pmc/articles/PMC4244143/ /pubmed/25422894 http://dx.doi.org/10.1371/journal.pone.0113668 Text en © 2014 Yin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yin, Yongtai
He, Yuchi
Liu, Wei
Gan, Lu
Fu, Chunhua
Jia, Haibo
Li, Maoteng
The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L
title The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L
title_full The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L
title_fullStr The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L
title_full_unstemmed The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L
title_short The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L
title_sort durative use of suspension cells and callus for volatile oil by comparative with seeds and fruits in capparis spinosa l
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244143/
https://www.ncbi.nlm.nih.gov/pubmed/25422894
http://dx.doi.org/10.1371/journal.pone.0113668
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