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Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress

In plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into the apoplast, are systemically mobile and elicit a range of responses signaling via cGMP. The PNP-dependent responses include tissue specific modific...

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Autores principales: Turek, Ilona, Marondedze, Claudius, Wheeler, Janet I., Gehring, Chris, Irving, Helen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244590/
https://www.ncbi.nlm.nih.gov/pubmed/25505478
http://dx.doi.org/10.3389/fpls.2014.00661
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author Turek, Ilona
Marondedze, Claudius
Wheeler, Janet I.
Gehring, Chris
Irving, Helen R.
author_facet Turek, Ilona
Marondedze, Claudius
Wheeler, Janet I.
Gehring, Chris
Irving, Helen R.
author_sort Turek, Ilona
collection PubMed
description In plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into the apoplast, are systemically mobile and elicit a range of responses signaling via cGMP. The PNP-dependent responses include tissue specific modifications of cation transport and changes in stomatal conductance and the photosynthetic rate. PNP also has a critical role in host defense responses. Surprisingly, PNP-homologs are produced by several plant pathogens during host colonization suppressing host defense responses. Here we show that a synthetic peptide representing the biologically active fragment of the Arabidopsis thaliana PNP (AtPNP-A) induces the production of reactive oxygen species in suspension-cultured A. thaliana (Col-0) cells. To identify proteins whose expression changes in an AtPNP-A dependent manner, we undertook a quantitative proteomic approach, employing tandem mass tag (TMT) labeling, to reveal temporal responses of suspension-cultured cells to 1 nM and 10 pM PNP at two different time-points post-treatment. Both concentrations yield a distinct differential proteome signature. Since only the higher (1 nM) concentration induces a ROS response, we conclude that the proteome response at the lower concentration reflects a ROS independent response. Furthermore, treatment with 1 nM PNP results in an over-representation of the gene ontology (GO) terms “oxidation-reduction process,” “translation” and “response to salt stress” and this is consistent with a role of AtPNP-A in the adaptation to environmental stress conditions.
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spelling pubmed-42445902014-12-10 Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress Turek, Ilona Marondedze, Claudius Wheeler, Janet I. Gehring, Chris Irving, Helen R. Front Plant Sci Plant Science In plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into the apoplast, are systemically mobile and elicit a range of responses signaling via cGMP. The PNP-dependent responses include tissue specific modifications of cation transport and changes in stomatal conductance and the photosynthetic rate. PNP also has a critical role in host defense responses. Surprisingly, PNP-homologs are produced by several plant pathogens during host colonization suppressing host defense responses. Here we show that a synthetic peptide representing the biologically active fragment of the Arabidopsis thaliana PNP (AtPNP-A) induces the production of reactive oxygen species in suspension-cultured A. thaliana (Col-0) cells. To identify proteins whose expression changes in an AtPNP-A dependent manner, we undertook a quantitative proteomic approach, employing tandem mass tag (TMT) labeling, to reveal temporal responses of suspension-cultured cells to 1 nM and 10 pM PNP at two different time-points post-treatment. Both concentrations yield a distinct differential proteome signature. Since only the higher (1 nM) concentration induces a ROS response, we conclude that the proteome response at the lower concentration reflects a ROS independent response. Furthermore, treatment with 1 nM PNP results in an over-representation of the gene ontology (GO) terms “oxidation-reduction process,” “translation” and “response to salt stress” and this is consistent with a role of AtPNP-A in the adaptation to environmental stress conditions. Frontiers Media S.A. 2014-11-26 /pmc/articles/PMC4244590/ /pubmed/25505478 http://dx.doi.org/10.3389/fpls.2014.00661 Text en Copyright © 2014 Turek, Marondedze, Wheeler, Gehring and Irving. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Turek, Ilona
Marondedze, Claudius
Wheeler, Janet I.
Gehring, Chris
Irving, Helen R.
Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
title Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
title_full Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
title_fullStr Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
title_full_unstemmed Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
title_short Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
title_sort plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244590/
https://www.ncbi.nlm.nih.gov/pubmed/25505478
http://dx.doi.org/10.3389/fpls.2014.00661
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