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Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays
Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This me...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244598/ https://www.ncbi.nlm.nih.gov/pubmed/25505484 http://dx.doi.org/10.3389/fgene.2014.00413 |