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Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis

In this study, we investigated the potential role of high-mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) and the effects of growth hormone (G) and somatostatin (S) in SAP rats. The rats were randomly divided into 6 groups of 20 each: sham-operated, SAP, SAP+saline, SAP+G, SAP+S and...

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Autores principales: Wang, Y.F., Wu, M., Ma, B.J., Cai, D.A., Yin, B.B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244674/
https://www.ncbi.nlm.nih.gov/pubmed/25387675
http://dx.doi.org/10.1590/1414-431X20143165
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author Wang, Y.F.
Wu, M.
Ma, B.J.
Cai, D.A.
Yin, B.B.
author_facet Wang, Y.F.
Wu, M.
Ma, B.J.
Cai, D.A.
Yin, B.B.
author_sort Wang, Y.F.
collection PubMed
description In this study, we investigated the potential role of high-mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) and the effects of growth hormone (G) and somatostatin (S) in SAP rats. The rats were randomly divided into 6 groups of 20 each: sham-operated, SAP, SAP+saline, SAP+G, SAP+S and SAP+G+S. Ileum and pancreas tissues of rats in each group were evaluated histologically. HMGB1 mRNA expression was measured by reverse transcription-PCR. Levels of circulating TNF-α, IL-1, IL-6, and endotoxin were also measured. In the SAP group, interstitial congestion and edema, inflammatory cell infiltration, and interstitial hemorrhage occurred in ileum and pancreas tissues. The levels of HMGB1, TNF-α, IL-1, IL-6 and endotoxin were significantly up-regulated in the SAP group compared with those in the sham-operated group, and the 7-day survival rate was 0%. In the SAP+G and SAP+S groups, the inflammatory response of the morphological structures was alleviated, the levels of HMGB1, TNF-α, IL-1, IL-6, and endotoxin were significantly decreased compared with those in the SAP group, and the survival rate was increased. Moreover, in the SAP+G+S group, all histological scores were significantly improved and the survival rate was significantly higher compared with the SAP group. In conclusion, HMGB1 might participate in pancreas and ileum injury in SAP. Growth hormone and somatostatin might play a therapeutic role in the inflammatory response of SAP.
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spelling pubmed-42446742014-12-08 Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis Wang, Y.F. Wu, M. Ma, B.J. Cai, D.A. Yin, B.B. Braz J Med Biol Res Biomedical Sciences In this study, we investigated the potential role of high-mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) and the effects of growth hormone (G) and somatostatin (S) in SAP rats. The rats were randomly divided into 6 groups of 20 each: sham-operated, SAP, SAP+saline, SAP+G, SAP+S and SAP+G+S. Ileum and pancreas tissues of rats in each group were evaluated histologically. HMGB1 mRNA expression was measured by reverse transcription-PCR. Levels of circulating TNF-α, IL-1, IL-6, and endotoxin were also measured. In the SAP group, interstitial congestion and edema, inflammatory cell infiltration, and interstitial hemorrhage occurred in ileum and pancreas tissues. The levels of HMGB1, TNF-α, IL-1, IL-6 and endotoxin were significantly up-regulated in the SAP group compared with those in the sham-operated group, and the 7-day survival rate was 0%. In the SAP+G and SAP+S groups, the inflammatory response of the morphological structures was alleviated, the levels of HMGB1, TNF-α, IL-1, IL-6, and endotoxin were significantly decreased compared with those in the SAP group, and the survival rate was increased. Moreover, in the SAP+G+S group, all histological scores were significantly improved and the survival rate was significantly higher compared with the SAP group. In conclusion, HMGB1 might participate in pancreas and ileum injury in SAP. Growth hormone and somatostatin might play a therapeutic role in the inflammatory response of SAP. Associação Brasileira de Divulgação Científica 2014-09-12 /pmc/articles/PMC4244674/ /pubmed/25387675 http://dx.doi.org/10.1590/1414-431X20143165 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedical Sciences
Wang, Y.F.
Wu, M.
Ma, B.J.
Cai, D.A.
Yin, B.B.
Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
title Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
title_full Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
title_fullStr Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
title_full_unstemmed Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
title_short Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
title_sort role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
topic Biomedical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244674/
https://www.ncbi.nlm.nih.gov/pubmed/25387675
http://dx.doi.org/10.1590/1414-431X20143165
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