Cargando…

Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium

The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe)...

Descripción completa

Detalles Bibliográficos
Autores principales: Flores Villavicencio, Lérida Liss, Cruz-Jiménez, Gustavo, Barbosa-Sabanero, Gloria, Kornhauser-Araujo, Carlos, Mendoza-Garrido, M. Eugenia, de la Rosa, Guadalupe, Sabanero-López, Myrna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244949/
https://www.ncbi.nlm.nih.gov/pubmed/25477771
http://dx.doi.org/10.1155/2014/923834
_version_ 1782346294905274368
author Flores Villavicencio, Lérida Liss
Cruz-Jiménez, Gustavo
Barbosa-Sabanero, Gloria
Kornhauser-Araujo, Carlos
Mendoza-Garrido, M. Eugenia
de la Rosa, Guadalupe
Sabanero-López, Myrna
author_facet Flores Villavicencio, Lérida Liss
Cruz-Jiménez, Gustavo
Barbosa-Sabanero, Gloria
Kornhauser-Araujo, Carlos
Mendoza-Garrido, M. Eugenia
de la Rosa, Guadalupe
Sabanero-López, Myrna
author_sort Flores Villavicencio, Lérida Liss
collection PubMed
description The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells.
format Online
Article
Text
id pubmed-4244949
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-42449492014-12-04 Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium Flores Villavicencio, Lérida Liss Cruz-Jiménez, Gustavo Barbosa-Sabanero, Gloria Kornhauser-Araujo, Carlos Mendoza-Garrido, M. Eugenia de la Rosa, Guadalupe Sabanero-López, Myrna Bioinorg Chem Appl Research Article The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells. Hindawi Publishing Corporation 2014 2014-11-12 /pmc/articles/PMC4244949/ /pubmed/25477771 http://dx.doi.org/10.1155/2014/923834 Text en Copyright © 2014 Lérida Liss Flores Villavicencio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Flores Villavicencio, Lérida Liss
Cruz-Jiménez, Gustavo
Barbosa-Sabanero, Gloria
Kornhauser-Araujo, Carlos
Mendoza-Garrido, M. Eugenia
de la Rosa, Guadalupe
Sabanero-López, Myrna
Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
title Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
title_full Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
title_fullStr Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
title_full_unstemmed Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
title_short Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
title_sort human lung cancer cell line a-549 atcc is differentially affected by supranutritional organic and inorganic selenium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244949/
https://www.ncbi.nlm.nih.gov/pubmed/25477771
http://dx.doi.org/10.1155/2014/923834
work_keys_str_mv AT floresvillavicencioleridaliss humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium
AT cruzjimenezgustavo humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium
AT barbosasabanerogloria humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium
AT kornhauseraraujocarlos humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium
AT mendozagarridomeugenia humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium
AT delarosaguadalupe humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium
AT sabanerolopezmyrna humanlungcancercelllinea549atccisdifferentiallyaffectedbysupranutritionalorganicandinorganicselenium