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Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe)...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244949/ https://www.ncbi.nlm.nih.gov/pubmed/25477771 http://dx.doi.org/10.1155/2014/923834 |
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author | Flores Villavicencio, Lérida Liss Cruz-Jiménez, Gustavo Barbosa-Sabanero, Gloria Kornhauser-Araujo, Carlos Mendoza-Garrido, M. Eugenia de la Rosa, Guadalupe Sabanero-López, Myrna |
author_facet | Flores Villavicencio, Lérida Liss Cruz-Jiménez, Gustavo Barbosa-Sabanero, Gloria Kornhauser-Araujo, Carlos Mendoza-Garrido, M. Eugenia de la Rosa, Guadalupe Sabanero-López, Myrna |
author_sort | Flores Villavicencio, Lérida Liss |
collection | PubMed |
description | The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells. |
format | Online Article Text |
id | pubmed-4244949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42449492014-12-04 Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium Flores Villavicencio, Lérida Liss Cruz-Jiménez, Gustavo Barbosa-Sabanero, Gloria Kornhauser-Araujo, Carlos Mendoza-Garrido, M. Eugenia de la Rosa, Guadalupe Sabanero-López, Myrna Bioinorg Chem Appl Research Article The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells. Hindawi Publishing Corporation 2014 2014-11-12 /pmc/articles/PMC4244949/ /pubmed/25477771 http://dx.doi.org/10.1155/2014/923834 Text en Copyright © 2014 Lérida Liss Flores Villavicencio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Flores Villavicencio, Lérida Liss Cruz-Jiménez, Gustavo Barbosa-Sabanero, Gloria Kornhauser-Araujo, Carlos Mendoza-Garrido, M. Eugenia de la Rosa, Guadalupe Sabanero-López, Myrna Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
title | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
title_full | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
title_fullStr | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
title_full_unstemmed | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
title_short | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
title_sort | human lung cancer cell line a-549 atcc is differentially affected by supranutritional organic and inorganic selenium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244949/ https://www.ncbi.nlm.nih.gov/pubmed/25477771 http://dx.doi.org/10.1155/2014/923834 |
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