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Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells
OBJECTIVE: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. METHODS: Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Obstetrics and Gynecology; Korean Society of Contraception and Reproductive Health; Korean Society of Gynecologic Endocrinology; Korean Society of Gynecologic Endoscopy and Minimal Invasive Surgery; Korean Society of Maternal Fetal Medicine; Korean Society of Ultrasound in Obstetrics and Gynecology; Korean Urogynecologic Society
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245344/ https://www.ncbi.nlm.nih.gov/pubmed/25469339 http://dx.doi.org/10.5468/ogs.2014.57.6.501 |
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author | Lee, Hyun Gyo Lee, Ji Min Shin, So Jin Kwon, Sang Hoon Lee, Gi Su Song, Chang Ho Choi, Eun Som Cha, Soon Do Cho, Chi Heum |
author_facet | Lee, Hyun Gyo Lee, Ji Min Shin, So Jin Kwon, Sang Hoon Lee, Gi Su Song, Chang Ho Choi, Eun Som Cha, Soon Do Cho, Chi Heum |
author_sort | Lee, Hyun Gyo |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. METHODS: Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. RESULTS: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. CONCLUSION: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma. |
format | Online Article Text |
id | pubmed-4245344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Obstetrics and Gynecology; Korean Society of Contraception and Reproductive Health; Korean Society of Gynecologic Endocrinology; Korean Society of Gynecologic Endoscopy and Minimal Invasive Surgery; Korean Society of Maternal Fetal Medicine; Korean Society of Ultrasound in Obstetrics and Gynecology; Korean Urogynecologic Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-42453442014-12-02 Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells Lee, Hyun Gyo Lee, Ji Min Shin, So Jin Kwon, Sang Hoon Lee, Gi Su Song, Chang Ho Choi, Eun Som Cha, Soon Do Cho, Chi Heum Obstet Gynecol Sci Original Article OBJECTIVE: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. METHODS: Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. RESULTS: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. CONCLUSION: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma. Korean Society of Obstetrics and Gynecology; Korean Society of Contraception and Reproductive Health; Korean Society of Gynecologic Endocrinology; Korean Society of Gynecologic Endoscopy and Minimal Invasive Surgery; Korean Society of Maternal Fetal Medicine; Korean Society of Ultrasound in Obstetrics and Gynecology; Korean Urogynecologic Society 2014-11 2014-11-20 /pmc/articles/PMC4245344/ /pubmed/25469339 http://dx.doi.org/10.5468/ogs.2014.57.6.501 Text en Copyright © 2014 Korean Society of Obstetrics and Gynecology http://creativecommons.org/licenses/by-nc/3.0/ Articles published in Obstet Gynecol Sci are open-access, distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Hyun Gyo Lee, Ji Min Shin, So Jin Kwon, Sang Hoon Lee, Gi Su Song, Chang Ho Choi, Eun Som Cha, Soon Do Cho, Chi Heum Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
title | Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
title_full | Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
title_fullStr | Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
title_full_unstemmed | Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
title_short | Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
title_sort | salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245344/ https://www.ncbi.nlm.nih.gov/pubmed/25469339 http://dx.doi.org/10.5468/ogs.2014.57.6.501 |
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