An Improved Surface Passivation Method for Single-Molecule Studies

We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also...

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Detalles Bibliográficos
Autores principales: Hua, Boyang, Young Han, Kyu, Zhou, Ruobo, Kim, Hajin, Shi, Xinghua, Abeysirigunawardena, Sanjaya C., Jain, Ankur, Singh, Digvijay, Aggarwal, Vasudha, Woodson, Sarah A., Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245390/
https://www.ncbi.nlm.nih.gov/pubmed/25306544
http://dx.doi.org/10.1038/nmeth.3143
Descripción
Sumario:We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution. Reduction in preparation time and cost is another major advantage.

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