An Improved Surface Passivation Method for Single-Molecule Studies

We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also...

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Autores principales: Hua, Boyang, Young Han, Kyu, Zhou, Ruobo, Kim, Hajin, Shi, Xinghua, Abeysirigunawardena, Sanjaya C., Jain, Ankur, Singh, Digvijay, Aggarwal, Vasudha, Woodson, Sarah A., Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245390/
https://www.ncbi.nlm.nih.gov/pubmed/25306544
http://dx.doi.org/10.1038/nmeth.3143
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author Hua, Boyang
Young Han, Kyu
Zhou, Ruobo
Kim, Hajin
Shi, Xinghua
Abeysirigunawardena, Sanjaya C.
Jain, Ankur
Singh, Digvijay
Aggarwal, Vasudha
Woodson, Sarah A.
Ha, Taekjip
author_facet Hua, Boyang
Young Han, Kyu
Zhou, Ruobo
Kim, Hajin
Shi, Xinghua
Abeysirigunawardena, Sanjaya C.
Jain, Ankur
Singh, Digvijay
Aggarwal, Vasudha
Woodson, Sarah A.
Ha, Taekjip
author_sort Hua, Boyang
collection PubMed
description We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution. Reduction in preparation time and cost is another major advantage.
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spelling pubmed-42453902015-06-01 An Improved Surface Passivation Method for Single-Molecule Studies Hua, Boyang Young Han, Kyu Zhou, Ruobo Kim, Hajin Shi, Xinghua Abeysirigunawardena, Sanjaya C. Jain, Ankur Singh, Digvijay Aggarwal, Vasudha Woodson, Sarah A. Ha, Taekjip Nat Methods Article We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution. Reduction in preparation time and cost is another major advantage. 2014-10-12 2014-12 /pmc/articles/PMC4245390/ /pubmed/25306544 http://dx.doi.org/10.1038/nmeth.3143 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hua, Boyang
Young Han, Kyu
Zhou, Ruobo
Kim, Hajin
Shi, Xinghua
Abeysirigunawardena, Sanjaya C.
Jain, Ankur
Singh, Digvijay
Aggarwal, Vasudha
Woodson, Sarah A.
Ha, Taekjip
An Improved Surface Passivation Method for Single-Molecule Studies
title An Improved Surface Passivation Method for Single-Molecule Studies
title_full An Improved Surface Passivation Method for Single-Molecule Studies
title_fullStr An Improved Surface Passivation Method for Single-Molecule Studies
title_full_unstemmed An Improved Surface Passivation Method for Single-Molecule Studies
title_short An Improved Surface Passivation Method for Single-Molecule Studies
title_sort improved surface passivation method for single-molecule studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245390/
https://www.ncbi.nlm.nih.gov/pubmed/25306544
http://dx.doi.org/10.1038/nmeth.3143
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