Cargando…

An Improved Surface Passivation Method for Single-Molecule Studies

We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hua, Boyang, Young Han, Kyu, Zhou, Ruobo, Kim, Hajin, Shi, Xinghua, Abeysirigunawardena, Sanjaya C., Jain, Ankur, Singh, Digvijay, Aggarwal, Vasudha, Woodson, Sarah A., Ha, Taekjip
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245390/ https://www.ncbi.nlm.nih.gov/pubmed/25306544 http://dx.doi.org/10.1038/nmeth.3143

Ejemplares similares

Protein-guided RNA dynamics during early ribosome assembly
por: Kim, Hajin, et al.
Publicado: (2014)

Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes
por: Abeysirigunawardena, Sanjaya C., et al.
Publicado: (2017)

Single molecule analysis of Thermus thermophilus SSB protein dynamics on single-stranded DNA
por: Zhang, Jichuan, et al.
Publicado: (2014)

Differential effects of ribosomal proteins and Mg(2+) ions on a conformational switch during 30S ribosome 5′-domain assembly
por: Abeysirigunawardena, Sanjaya C., et al.
Publicado: (2015)

Metals induce transient folding and activation of the Twister ribozyme
por: Panja, Subrata, et al.
Publicado: (2017)

Acidification of the Oxygen Scavenging System in Single-Molecule Fluorescence Studies: In Situ Sensing with a Ratiometric Dual-Emission Probe
por: Shi, Xinghua, et al.
Publicado: (2010)

Surface Passivation for Single-molecule Protein Studies
por: Chandradoss, Stanley D., et al.
Publicado: (2014)

A dendritic single-molecule fluorescent probe that is monovalent, photostable, and minimally blinking
por: Yang, Si Kyung, et al.
Publicado: (2013)

Impact of Small Molecules on Intermolecular G-Quadruplex Formation
por: Gyawali, Prabesh, et al.
Publicado: (2019)

Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
por: Okafor, Ikenna C, et al.
Publicado: (2019)

Probing Cellular Protein Complexes via Single Molecule Pull-down
por: Jain, Ankur, et al.
Publicado: (2011)

Mind your tag in single-molecule measurements
por: Merino Urteaga, Raquel, et al.
Publicado: (2023)

Quantitative Fluorescent Labeling of Aldehyde-Tagged Proteins for Single-Molecule Imaging
por: Shi, Xinghua, et al.
Publicado: (2012)

A rule of seven in Watson-Crick base pairing of mismatched sequences
por: Cisse, Ibrahim I., et al.
Publicado: (2012)

Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics
por: Okafor, Ikenna C., et al.
Publicado: (2022)

Direct evidence for sequence-dependent attraction between double-stranded DNA controlled by methylation
por: Yoo, Jejoong, et al.
Publicado: (2016)

A parameter estimation method for fluorescence lifetime data
por: Sewell, Daniel, et al.
Publicado: (2015)

Impact of Molecule Concentration, Diffusion Rates and Surface Passivation on Single-Molecule Fluorescence Studies in Solution
por: Yukhnovets, Olessya, et al.
Publicado: (2022)

Opening–closing dynamics of the mitochondrial transcription pre-initiation complex
por: Kim, Hajin, et al.
Publicado: (2012)

Surface Passivation with a Perfluoroalkane Brush Improves the Precision of Single-Molecule Measurements
por: Bueno-Alejo, Carlos J., et al.
Publicado: (2022)

Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17
por: Jayalath, Kumudie, et al.
Publicado: (2020)

Discovery of a novel small molecular peptide that disrupts helix 34 of bacterial ribosomal RNA
por: GC, Keshav, et al.
Publicado: (2019)

Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA
por: Zhou, Ruobo, et al.
Publicado: (2014)

Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase
por: Syed, Salman, et al.
Publicado: (2014)

Single molecule analysis reveals three phases of DNA degradation by an exonuclease
por: Lee, Gwangrog, et al.
Publicado: (2011)

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
por: Singh, Digvijay, et al.
Publicado: (2016)

A metastable rRNA junction essential for bacterial 30S biogenesis
por: Sharma, Indra Mani, et al.
Publicado: (2018)

The Telomere Capping Complex CST Has an Unusual Stoichiometry, Makes Multipartite Interaction with G-Tails, and Unfolds Higher-Order G-Tail Structures
por: Lue, Neal F., et al.
Publicado: (2013)

A Coarse-Grained Model of Unstructured Single-Stranded DNA Derived from Atomistic Simulation and Single-Molecule Experiment
por: Maffeo, Christopher, et al.
Publicado: (2014)

RsmG forms stable complexes with premature small subunit rRNA during bacterial ribosome biogenesis
por: Abedeera, Sudeshi M., et al.
Publicado: (2020)

Single Molecule Nanocontainers Made Porous Using a Bacterial Toxin
por: Okumus, Burak, et al.
Publicado: (2009)

Structural dynamics of potassium channel gating revealed by single molecule FRET
por: Wang, Shizhen, et al.
Publicado: (2015)

Interrogating accessibility of telomeric sequences with FRET-PAINT: evidence for length-dependent telomere compaction
por: Mustafa, Golam, et al.
Publicado: (2021)

Unraveling helicase mechanisms one molecule at a time
por: Rasnik, Ivan, et al.
Publicado: (2006)

The START domain potentiates HD-ZIPIII transcriptional activity
por: Husbands, Aman Y, et al.
Publicado: (2023)

Dynamics of Major Histocompatibility Complex Class I Association with the Human Peptide-loading Complex
por: Panter, Michaela S., et al.
Publicado: (2012)

Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level
por: Liao, Ting-Wei, et al.
Publicado: (2023)

Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress
por: Abedeera, Sudeshi M., et al.
Publicado: (2023)

Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
por: Hua, Boyang, et al.
Publicado: (2020)

Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
por: Paul, Tapas, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_i0t6fammv2rj9lgh7s43um2032