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Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles

[Image: see text] Ribosomes are molecular machines that function in polyribosome complexes to translate genetic information, guide the synthesis of polypeptides, and modulate the folding of nascent proteins. Here, we report a surprising function for polyribosomes as a result of a systematic examinat...

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Autores principales: Mrazek, Jan, Toso, Daniel, Ryazantsev, Sergey, Zhang, Xing, Zhou, Z. Hong, Fernandez, Beatriz Campo, Kickhoefer, Valerie A., Rome, Leonard H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245718/
https://www.ncbi.nlm.nih.gov/pubmed/25354757
http://dx.doi.org/10.1021/nn504778h
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author Mrazek, Jan
Toso, Daniel
Ryazantsev, Sergey
Zhang, Xing
Zhou, Z. Hong
Fernandez, Beatriz Campo
Kickhoefer, Valerie A.
Rome, Leonard H.
author_facet Mrazek, Jan
Toso, Daniel
Ryazantsev, Sergey
Zhang, Xing
Zhou, Z. Hong
Fernandez, Beatriz Campo
Kickhoefer, Valerie A.
Rome, Leonard H.
author_sort Mrazek, Jan
collection PubMed
description [Image: see text] Ribosomes are molecular machines that function in polyribosome complexes to translate genetic information, guide the synthesis of polypeptides, and modulate the folding of nascent proteins. Here, we report a surprising function for polyribosomes as a result of a systematic examination of the assembly of a large ribonucleoprotein complex, the vault particle. Structural and functional evidence points to a model of vault assembly whereby the polyribosome acts like a 3D nanoprinter to direct the ordered translation and assembly of the multi-subunit vault homopolymer, a process which we refer to as polyribosome templating. Structure-based mutagenesis and cell-free in vitro expression studies further demonstrated the critical importance of the polyribosome in vault assembly. Polyribosome templating prevents chaos by ensuring efficiency and order in the production of large homopolymeric protein structures in the crowded cellular environment and might explain the origin of many polyribosome-associated molecular assemblies inside the cell.
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spelling pubmed-42457182014-12-01 Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles Mrazek, Jan Toso, Daniel Ryazantsev, Sergey Zhang, Xing Zhou, Z. Hong Fernandez, Beatriz Campo Kickhoefer, Valerie A. Rome, Leonard H. ACS Nano [Image: see text] Ribosomes are molecular machines that function in polyribosome complexes to translate genetic information, guide the synthesis of polypeptides, and modulate the folding of nascent proteins. Here, we report a surprising function for polyribosomes as a result of a systematic examination of the assembly of a large ribonucleoprotein complex, the vault particle. Structural and functional evidence points to a model of vault assembly whereby the polyribosome acts like a 3D nanoprinter to direct the ordered translation and assembly of the multi-subunit vault homopolymer, a process which we refer to as polyribosome templating. Structure-based mutagenesis and cell-free in vitro expression studies further demonstrated the critical importance of the polyribosome in vault assembly. Polyribosome templating prevents chaos by ensuring efficiency and order in the production of large homopolymeric protein structures in the crowded cellular environment and might explain the origin of many polyribosome-associated molecular assemblies inside the cell. American Chemical Society 2014-10-30 2014-11-25 /pmc/articles/PMC4245718/ /pubmed/25354757 http://dx.doi.org/10.1021/nn504778h Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Mrazek, Jan
Toso, Daniel
Ryazantsev, Sergey
Zhang, Xing
Zhou, Z. Hong
Fernandez, Beatriz Campo
Kickhoefer, Valerie A.
Rome, Leonard H.
Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
title Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
title_full Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
title_fullStr Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
title_full_unstemmed Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
title_short Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
title_sort polyribosomes are molecular 3d nanoprinters that orchestrate the assembly of vault particles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245718/
https://www.ncbi.nlm.nih.gov/pubmed/25354757
http://dx.doi.org/10.1021/nn504778h
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