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Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay

Key Concepts: The two-dimensional Two-Tailed Comet assay (TT-comet) protocol is a valuable technique to differentiate between single-stranded (SSBs) and double-stranded DNA breaks (DSBs) on the same sperm cell. Protein lysis inherent with the TT-comet protocol accounts for differences in sperm prota...

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Detalles Bibliográficos
Autores principales:	Cortés-Gutiérrez, Elva I., López-Fernández, Carmen, Fernández, José Luis, Dávila-Rodríguez, Martha I., Johnston, Stephen D., Gosálvez, Jaime
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2014
Materias:	Genetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245925/ https://www.ncbi.nlm.nih.gov/pubmed/25505901 http://dx.doi.org/10.3389/fgene.2014.00404

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