Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair

[Image: see text] We recently showed that Watson–Crick base pairs in canonical duplex DNA exist in dynamic equilibrium with G(syn)·C(+) and A(syn)·T Hoogsteen base pairs that have minute populations of ∼1%. Here, using nuclear magnetic resonance R(1ρ) relaxation dispersion, we show that substitution...

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Autores principales: Nikolova, Evgenia N., Stull, Frederick, Al-Hashimi, Hashim M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245982/
https://www.ncbi.nlm.nih.gov/pubmed/25339065
http://dx.doi.org/10.1021/bi5011909
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author Nikolova, Evgenia N.
Stull, Frederick
Al-Hashimi, Hashim M.
author_facet Nikolova, Evgenia N.
Stull, Frederick
Al-Hashimi, Hashim M.
author_sort Nikolova, Evgenia N.
collection PubMed
description [Image: see text] We recently showed that Watson–Crick base pairs in canonical duplex DNA exist in dynamic equilibrium with G(syn)·C(+) and A(syn)·T Hoogsteen base pairs that have minute populations of ∼1%. Here, using nuclear magnetic resonance R(1ρ) relaxation dispersion, we show that substitution of guanine with the naturally occurring base inosine results in an ∼17-fold increase in the population of transient Hoogsteen base pairs, which can be rationalized by the loss of a Watson–Crick hydrogen bond. These results provide further support for transient Hoogsteen base pairs and demonstrate that their population can increase significantly upon damage or chemical modification of the base.
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spelling pubmed-42459822015-10-23 Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair Nikolova, Evgenia N. Stull, Frederick Al-Hashimi, Hashim M. Biochemistry [Image: see text] We recently showed that Watson–Crick base pairs in canonical duplex DNA exist in dynamic equilibrium with G(syn)·C(+) and A(syn)·T Hoogsteen base pairs that have minute populations of ∼1%. Here, using nuclear magnetic resonance R(1ρ) relaxation dispersion, we show that substitution of guanine with the naturally occurring base inosine results in an ∼17-fold increase in the population of transient Hoogsteen base pairs, which can be rationalized by the loss of a Watson–Crick hydrogen bond. These results provide further support for transient Hoogsteen base pairs and demonstrate that their population can increase significantly upon damage or chemical modification of the base. American Chemical Society 2014-10-23 2014-11-25 /pmc/articles/PMC4245982/ /pubmed/25339065 http://dx.doi.org/10.1021/bi5011909 Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Nikolova, Evgenia N.
Stull, Frederick
Al-Hashimi, Hashim M.
Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair
title Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair
title_full Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair
title_fullStr Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair
title_full_unstemmed Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair
title_short Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair
title_sort guanine to inosine substitution leads to large increases in the population of a transient g·c hoogsteen base pair
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245982/
https://www.ncbi.nlm.nih.gov/pubmed/25339065
http://dx.doi.org/10.1021/bi5011909
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AT alhashimihashimm guaninetoinosinesubstitutionleadstolargeincreasesinthepopulationofatransientgchoogsteenbasepair

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