Cargando…
WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce
Proper embryo development is crucial as that is when the primary body axes are established. In Arabidopsis, AtWOX8 and AtWOX9, members of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, are critical for embryo development. In Norway spruce, PaWOX8/9, which is expressed in embryos, is the homologue o...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246185/ https://www.ncbi.nlm.nih.gov/pubmed/25205582 http://dx.doi.org/10.1093/jxb/eru371 |
_version_ | 1782346485667463168 |
---|---|
author | Zhu, Tianqing Moschou, Panagiotis N. Alvarez, José M. Sohlberg, Joel J. von Arnold, Sara |
author_facet | Zhu, Tianqing Moschou, Panagiotis N. Alvarez, José M. Sohlberg, Joel J. von Arnold, Sara |
author_sort | Zhu, Tianqing |
collection | PubMed |
description | Proper embryo development is crucial as that is when the primary body axes are established. In Arabidopsis, AtWOX8 and AtWOX9, members of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, are critical for embryo development. In Norway spruce, PaWOX8/9, which is expressed in embryos, is the homologue of AtWOX8 and AtWOX9. In this work, it is shown that the transcript abundance of PaWOX8/9 is high during early and late embryogeny and that it decreases when the maturation phase starts. To address the function of PaWOX8/9 during embryo development, RNAi lines were established to down-regulate the transcript level of PaWOX8/9, using both constitutive and inducible promoters. Embryos in the PaWOX8/9 RNAi lines show an aberrant morphology caused by disturbed orientation of the cell division plane at the basal part of the embryonal mass during early and late embryogeny. In addition, the transcript level of several key cell-cycle-regulating genes, for example, PaE2FAB-LIKE and PaCYCLIN B-LIKE, are affected in the PaWOX8/9 RNAi lines. Taken together, our results suggest that PaWOX8/9 may perform an evolutionarily conserved function as a regulator of the establishment of the apical–basal embryo pattern. |
format | Online Article Text |
id | pubmed-4246185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-42461852014-12-04 WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce Zhu, Tianqing Moschou, Panagiotis N. Alvarez, José M. Sohlberg, Joel J. von Arnold, Sara J Exp Bot Research Paper Proper embryo development is crucial as that is when the primary body axes are established. In Arabidopsis, AtWOX8 and AtWOX9, members of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, are critical for embryo development. In Norway spruce, PaWOX8/9, which is expressed in embryos, is the homologue of AtWOX8 and AtWOX9. In this work, it is shown that the transcript abundance of PaWOX8/9 is high during early and late embryogeny and that it decreases when the maturation phase starts. To address the function of PaWOX8/9 during embryo development, RNAi lines were established to down-regulate the transcript level of PaWOX8/9, using both constitutive and inducible promoters. Embryos in the PaWOX8/9 RNAi lines show an aberrant morphology caused by disturbed orientation of the cell division plane at the basal part of the embryonal mass during early and late embryogeny. In addition, the transcript level of several key cell-cycle-regulating genes, for example, PaE2FAB-LIKE and PaCYCLIN B-LIKE, are affected in the PaWOX8/9 RNAi lines. Taken together, our results suggest that PaWOX8/9 may perform an evolutionarily conserved function as a regulator of the establishment of the apical–basal embryo pattern. Oxford University Press 2014-12 2014-09-09 /pmc/articles/PMC4246185/ /pubmed/25205582 http://dx.doi.org/10.1093/jxb/eru371 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Zhu, Tianqing Moschou, Panagiotis N. Alvarez, José M. Sohlberg, Joel J. von Arnold, Sara WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce |
title |
WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce |
title_full |
WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce |
title_fullStr |
WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce |
title_full_unstemmed |
WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce |
title_short |
WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce |
title_sort | wuschel-related homeobox 8/9 is important for proper embryo patterning in the gymnosperm norway spruce |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246185/ https://www.ncbi.nlm.nih.gov/pubmed/25205582 http://dx.doi.org/10.1093/jxb/eru371 |
work_keys_str_mv | AT zhutianqing wuschelrelatedhomeobox89isimportantforproperembryopatterninginthegymnospermnorwayspruce AT moschoupanagiotisn wuschelrelatedhomeobox89isimportantforproperembryopatterninginthegymnospermnorwayspruce AT alvarezjosem wuschelrelatedhomeobox89isimportantforproperembryopatterninginthegymnospermnorwayspruce AT sohlbergjoelj wuschelrelatedhomeobox89isimportantforproperembryopatterninginthegymnospermnorwayspruce AT vonarnoldsara wuschelrelatedhomeobox89isimportantforproperembryopatterninginthegymnospermnorwayspruce |