Cargando…
Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
Multiple products are being developed for use against filoviral infections. Efficacy for these products will likely be demonstrated in nonhuman primate models of filoviral disease to satisfy licensure requirements under the Animal Rule, or to supplement human data. Typically, the endpoint for effica...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246243/ https://www.ncbi.nlm.nih.gov/pubmed/25421892 http://dx.doi.org/10.3390/v6114666 |
_version_ | 1782346498346844160 |
---|---|
author | Warren, Travis K. Trefry, John C. Marko, Shannon T. Chance, Taylor B. Wells, Jay B. Pratt, William D. Johnson, Joshua C. Mucker, Eric M. Norris, Sarah L. Chappell, Mark Dye, John M. Honko, Anna N. |
author_facet | Warren, Travis K. Trefry, John C. Marko, Shannon T. Chance, Taylor B. Wells, Jay B. Pratt, William D. Johnson, Joshua C. Mucker, Eric M. Norris, Sarah L. Chappell, Mark Dye, John M. Honko, Anna N. |
author_sort | Warren, Travis K. |
collection | PubMed |
description | Multiple products are being developed for use against filoviral infections. Efficacy for these products will likely be demonstrated in nonhuman primate models of filoviral disease to satisfy licensure requirements under the Animal Rule, or to supplement human data. Typically, the endpoint for efficacy assessment will be survival following challenge; however, there exists no standardized approach for assessing the health or euthanasia criteria for filovirus-exposed nonhuman primates. Consideration of objective criteria is important to (a) ensure test subjects are euthanized without unnecessary distress; (b) enhance the likelihood that animals exhibiting mild or moderate signs of disease are not prematurely euthanized; (c) minimize the occurrence of spontaneous deaths and loss of end-stage samples; (d) enhance the reproducibility of experiments between different researchers; and (e) provide a defensible rationale for euthanasia decisions that withstands regulatory scrutiny. Historic records were compiled for 58 surviving and non-surviving monkeys exposed to Ebola virus at the US Army Medical Research Institute of Infectious Diseases. Clinical pathology parameters were statistically analyzed and those exhibiting predicative value for survival are reported. These findings may be useful for standardization of objective euthanasia assessments in rhesus monkeys exposed to Ebola virus and may serve as a useful approach for other standardization efforts. |
format | Online Article Text |
id | pubmed-4246243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42462432014-12-01 Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates Warren, Travis K. Trefry, John C. Marko, Shannon T. Chance, Taylor B. Wells, Jay B. Pratt, William D. Johnson, Joshua C. Mucker, Eric M. Norris, Sarah L. Chappell, Mark Dye, John M. Honko, Anna N. Viruses Article Multiple products are being developed for use against filoviral infections. Efficacy for these products will likely be demonstrated in nonhuman primate models of filoviral disease to satisfy licensure requirements under the Animal Rule, or to supplement human data. Typically, the endpoint for efficacy assessment will be survival following challenge; however, there exists no standardized approach for assessing the health or euthanasia criteria for filovirus-exposed nonhuman primates. Consideration of objective criteria is important to (a) ensure test subjects are euthanized without unnecessary distress; (b) enhance the likelihood that animals exhibiting mild or moderate signs of disease are not prematurely euthanized; (c) minimize the occurrence of spontaneous deaths and loss of end-stage samples; (d) enhance the reproducibility of experiments between different researchers; and (e) provide a defensible rationale for euthanasia decisions that withstands regulatory scrutiny. Historic records were compiled for 58 surviving and non-surviving monkeys exposed to Ebola virus at the US Army Medical Research Institute of Infectious Diseases. Clinical pathology parameters were statistically analyzed and those exhibiting predicative value for survival are reported. These findings may be useful for standardization of objective euthanasia assessments in rhesus monkeys exposed to Ebola virus and may serve as a useful approach for other standardization efforts. MDPI 2014-11-24 /pmc/articles/PMC4246243/ /pubmed/25421892 http://dx.doi.org/10.3390/v6114666 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Warren, Travis K. Trefry, John C. Marko, Shannon T. Chance, Taylor B. Wells, Jay B. Pratt, William D. Johnson, Joshua C. Mucker, Eric M. Norris, Sarah L. Chappell, Mark Dye, John M. Honko, Anna N. Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates |
title | Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates |
title_full | Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates |
title_fullStr | Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates |
title_full_unstemmed | Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates |
title_short | Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates |
title_sort | euthanasia assessment in ebola virus infected nonhuman primates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246243/ https://www.ncbi.nlm.nih.gov/pubmed/25421892 http://dx.doi.org/10.3390/v6114666 |
work_keys_str_mv | AT warrentravisk euthanasiaassessmentinebolavirusinfectednonhumanprimates AT trefryjohnc euthanasiaassessmentinebolavirusinfectednonhumanprimates AT markoshannont euthanasiaassessmentinebolavirusinfectednonhumanprimates AT chancetaylorb euthanasiaassessmentinebolavirusinfectednonhumanprimates AT wellsjayb euthanasiaassessmentinebolavirusinfectednonhumanprimates AT prattwilliamd euthanasiaassessmentinebolavirusinfectednonhumanprimates AT johnsonjoshuac euthanasiaassessmentinebolavirusinfectednonhumanprimates AT muckerericm euthanasiaassessmentinebolavirusinfectednonhumanprimates AT norrissarahl euthanasiaassessmentinebolavirusinfectednonhumanprimates AT chappellmark euthanasiaassessmentinebolavirusinfectednonhumanprimates AT dyejohnm euthanasiaassessmentinebolavirusinfectednonhumanprimates AT honkoannan euthanasiaassessmentinebolavirusinfectednonhumanprimates |