Cargando…

Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates

Multiple products are being developed for use against filoviral infections. Efficacy for these products will likely be demonstrated in nonhuman primate models of filoviral disease to satisfy licensure requirements under the Animal Rule, or to supplement human data. Typically, the endpoint for effica...

Descripción completa

Detalles Bibliográficos
Autores principales: Warren, Travis K., Trefry, John C., Marko, Shannon T., Chance, Taylor B., Wells, Jay B., Pratt, William D., Johnson, Joshua C., Mucker, Eric M., Norris, Sarah L., Chappell, Mark, Dye, John M., Honko, Anna N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246243/
https://www.ncbi.nlm.nih.gov/pubmed/25421892
http://dx.doi.org/10.3390/v6114666
_version_ 1782346498346844160
author Warren, Travis K.
Trefry, John C.
Marko, Shannon T.
Chance, Taylor B.
Wells, Jay B.
Pratt, William D.
Johnson, Joshua C.
Mucker, Eric M.
Norris, Sarah L.
Chappell, Mark
Dye, John M.
Honko, Anna N.
author_facet Warren, Travis K.
Trefry, John C.
Marko, Shannon T.
Chance, Taylor B.
Wells, Jay B.
Pratt, William D.
Johnson, Joshua C.
Mucker, Eric M.
Norris, Sarah L.
Chappell, Mark
Dye, John M.
Honko, Anna N.
author_sort Warren, Travis K.
collection PubMed
description Multiple products are being developed for use against filoviral infections. Efficacy for these products will likely be demonstrated in nonhuman primate models of filoviral disease to satisfy licensure requirements under the Animal Rule, or to supplement human data. Typically, the endpoint for efficacy assessment will be survival following challenge; however, there exists no standardized approach for assessing the health or euthanasia criteria for filovirus-exposed nonhuman primates. Consideration of objective criteria is important to (a) ensure test subjects are euthanized without unnecessary distress; (b) enhance the likelihood that animals exhibiting mild or moderate signs of disease are not prematurely euthanized; (c) minimize the occurrence of spontaneous deaths and loss of end-stage samples; (d) enhance the reproducibility of experiments between different researchers; and (e) provide a defensible rationale for euthanasia decisions that withstands regulatory scrutiny. Historic records were compiled for 58 surviving and non-surviving monkeys exposed to Ebola virus at the US Army Medical Research Institute of Infectious Diseases. Clinical pathology parameters were statistically analyzed and those exhibiting predicative value for survival are reported. These findings may be useful for standardization of objective euthanasia assessments in rhesus monkeys exposed to Ebola virus and may serve as a useful approach for other standardization efforts.
format Online
Article
Text
id pubmed-4246243
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42462432014-12-01 Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates Warren, Travis K. Trefry, John C. Marko, Shannon T. Chance, Taylor B. Wells, Jay B. Pratt, William D. Johnson, Joshua C. Mucker, Eric M. Norris, Sarah L. Chappell, Mark Dye, John M. Honko, Anna N. Viruses Article Multiple products are being developed for use against filoviral infections. Efficacy for these products will likely be demonstrated in nonhuman primate models of filoviral disease to satisfy licensure requirements under the Animal Rule, or to supplement human data. Typically, the endpoint for efficacy assessment will be survival following challenge; however, there exists no standardized approach for assessing the health or euthanasia criteria for filovirus-exposed nonhuman primates. Consideration of objective criteria is important to (a) ensure test subjects are euthanized without unnecessary distress; (b) enhance the likelihood that animals exhibiting mild or moderate signs of disease are not prematurely euthanized; (c) minimize the occurrence of spontaneous deaths and loss of end-stage samples; (d) enhance the reproducibility of experiments between different researchers; and (e) provide a defensible rationale for euthanasia decisions that withstands regulatory scrutiny. Historic records were compiled for 58 surviving and non-surviving monkeys exposed to Ebola virus at the US Army Medical Research Institute of Infectious Diseases. Clinical pathology parameters were statistically analyzed and those exhibiting predicative value for survival are reported. These findings may be useful for standardization of objective euthanasia assessments in rhesus monkeys exposed to Ebola virus and may serve as a useful approach for other standardization efforts. MDPI 2014-11-24 /pmc/articles/PMC4246243/ /pubmed/25421892 http://dx.doi.org/10.3390/v6114666 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Warren, Travis K.
Trefry, John C.
Marko, Shannon T.
Chance, Taylor B.
Wells, Jay B.
Pratt, William D.
Johnson, Joshua C.
Mucker, Eric M.
Norris, Sarah L.
Chappell, Mark
Dye, John M.
Honko, Anna N.
Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
title Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
title_full Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
title_fullStr Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
title_full_unstemmed Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
title_short Euthanasia Assessment in Ebola Virus Infected Nonhuman Primates
title_sort euthanasia assessment in ebola virus infected nonhuman primates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246243/
https://www.ncbi.nlm.nih.gov/pubmed/25421892
http://dx.doi.org/10.3390/v6114666
work_keys_str_mv AT warrentravisk euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT trefryjohnc euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT markoshannont euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT chancetaylorb euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT wellsjayb euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT prattwilliamd euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT johnsonjoshuac euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT muckerericm euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT norrissarahl euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT chappellmark euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT dyejohnm euthanasiaassessmentinebolavirusinfectednonhumanprimates
AT honkoannan euthanasiaassessmentinebolavirusinfectednonhumanprimates