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A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing
BACKGROUND: CRISPR-Cas9 is a revolutionary genome editing technique that allows for efficient and directed alterations of the eukaryotic genome. This relatively new technology has already been used in a large number of ‘loss of function’ experiments in cultured cells. Despite its simplicity and effi...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246457/ https://www.ncbi.nlm.nih.gov/pubmed/25409780 http://dx.doi.org/10.1186/1471-2164-15-1002 |