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Isolation, cultivation and identification of human lung adenocarcinoma stem cells

Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133(+) phenotype wa...

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Autores principales: ZHANG, DE-GENG, JIANG, AI-GUI, LU, HUI-YU, ZHANG, LI-XIN, GAO, XIAO-YAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246672/
https://www.ncbi.nlm.nih.gov/pubmed/25435932
http://dx.doi.org/10.3892/ol.2014.2639
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author ZHANG, DE-GENG
JIANG, AI-GUI
LU, HUI-YU
ZHANG, LI-XIN
GAO, XIAO-YAN
author_facet ZHANG, DE-GENG
JIANG, AI-GUI
LU, HUI-YU
ZHANG, LI-XIN
GAO, XIAO-YAN
author_sort ZHANG, DE-GENG
collection PubMed
description Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133(+) phenotype was successfully isolated from a single cell suspension of lung adenocarcinoma tissue using magnetic-activated cell sorting (MACS) and enriched in a serum-free culture. In comparison to CD133(−) cells, the CD133(+) cells exhibited an enhanced capacity for self-renewal and differentiation, and a greater potential for in vivo tumor formation, in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Tumors could be induced in NOD/SCID mice by the transplantation of 10(2) stem-like cells per mouse. The results of the present study demonstrated that CD133 may serve as a specific cell surface marker for lung adenocarcinoma stem cells, and that MACS combined with serum-free culture is an effective method for isolating and enriching lung cancer stem cells.
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spelling pubmed-42466722014-11-28 Isolation, cultivation and identification of human lung adenocarcinoma stem cells ZHANG, DE-GENG JIANG, AI-GUI LU, HUI-YU ZHANG, LI-XIN GAO, XIAO-YAN Oncol Lett Articles Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133(+) phenotype was successfully isolated from a single cell suspension of lung adenocarcinoma tissue using magnetic-activated cell sorting (MACS) and enriched in a serum-free culture. In comparison to CD133(−) cells, the CD133(+) cells exhibited an enhanced capacity for self-renewal and differentiation, and a greater potential for in vivo tumor formation, in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Tumors could be induced in NOD/SCID mice by the transplantation of 10(2) stem-like cells per mouse. The results of the present study demonstrated that CD133 may serve as a specific cell surface marker for lung adenocarcinoma stem cells, and that MACS combined with serum-free culture is an effective method for isolating and enriching lung cancer stem cells. D.A. Spandidos 2015-01 2014-10-24 /pmc/articles/PMC4246672/ /pubmed/25435932 http://dx.doi.org/10.3892/ol.2014.2639 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHANG, DE-GENG
JIANG, AI-GUI
LU, HUI-YU
ZHANG, LI-XIN
GAO, XIAO-YAN
Isolation, cultivation and identification of human lung adenocarcinoma stem cells
title Isolation, cultivation and identification of human lung adenocarcinoma stem cells
title_full Isolation, cultivation and identification of human lung adenocarcinoma stem cells
title_fullStr Isolation, cultivation and identification of human lung adenocarcinoma stem cells
title_full_unstemmed Isolation, cultivation and identification of human lung adenocarcinoma stem cells
title_short Isolation, cultivation and identification of human lung adenocarcinoma stem cells
title_sort isolation, cultivation and identification of human lung adenocarcinoma stem cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246672/
https://www.ncbi.nlm.nih.gov/pubmed/25435932
http://dx.doi.org/10.3892/ol.2014.2639
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