Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

BACKGROUND: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR). FINDINGS: In total, 15 RT-qPCR positi...

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Autores principales: Fischer, Melina, Freuling, Conrad M, Müller, Thomas, Schatz, Juliane, Rasmussen, Thomas Bruun, Chriel, Mariann, Balkema-Buschmann, Anne, Beer, Martin, Hoffmann, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4247638/
https://www.ncbi.nlm.nih.gov/pubmed/25420461
http://dx.doi.org/10.1186/s12985-014-0196-x
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author Fischer, Melina
Freuling, Conrad M
Müller, Thomas
Schatz, Juliane
Rasmussen, Thomas Bruun
Chriel, Mariann
Balkema-Buschmann, Anne
Beer, Martin
Hoffmann, Bernd
author_facet Fischer, Melina
Freuling, Conrad M
Müller, Thomas
Schatz, Juliane
Rasmussen, Thomas Bruun
Chriel, Mariann
Balkema-Buschmann, Anne
Beer, Martin
Hoffmann, Bernd
author_sort Fischer, Melina
collection PubMed
description BACKGROUND: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR). FINDINGS: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. CONCLUSIONS: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.
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spelling pubmed-42476382014-11-30 Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats Fischer, Melina Freuling, Conrad M Müller, Thomas Schatz, Juliane Rasmussen, Thomas Bruun Chriel, Mariann Balkema-Buschmann, Anne Beer, Martin Hoffmann, Bernd Virol J Short Report BACKGROUND: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR). FINDINGS: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. CONCLUSIONS: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae. BioMed Central 2014-11-25 /pmc/articles/PMC4247638/ /pubmed/25420461 http://dx.doi.org/10.1186/s12985-014-0196-x Text en © Fischer et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Fischer, Melina
Freuling, Conrad M
Müller, Thomas
Schatz, Juliane
Rasmussen, Thomas Bruun
Chriel, Mariann
Balkema-Buschmann, Anne
Beer, Martin
Hoffmann, Bernd
Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
title Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
title_full Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
title_fullStr Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
title_full_unstemmed Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
title_short Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
title_sort identification of rhabdoviral sequences in oropharyngeal swabs from german and danish bats
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4247638/
https://www.ncbi.nlm.nih.gov/pubmed/25420461
http://dx.doi.org/10.1186/s12985-014-0196-x
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