Cargando…

The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation

Background: Despite extensive progress in IVF techniques, one of the most difficult problems is the variability in the response to controlled ovarian hyperstimulation (COH). Recent studies show the effects of individual genetic variability on COH outcome. Objective: To evaluate the correlation betwe...

Descripción completa

Detalles Bibliográficos
Autores principales: Davar, Robab, Tabibnejad, Nasim, Kalantar, Seyed Mehdi, Sheikhha, Mohammad Hasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Clinical Center for Infertility 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248152/
https://www.ncbi.nlm.nih.gov/pubmed/25469124
_version_ 1782346759476871168
author Davar, Robab
Tabibnejad, Nasim
Kalantar, Seyed Mehdi
Sheikhha, Mohammad Hasan
author_facet Davar, Robab
Tabibnejad, Nasim
Kalantar, Seyed Mehdi
Sheikhha, Mohammad Hasan
author_sort Davar, Robab
collection PubMed
description Background: Despite extensive progress in IVF techniques, one of the most difficult problems is the variability in the response to controlled ovarian hyperstimulation (COH). Recent studies show the effects of individual genetic variability on COH outcome. Objective: To evaluate the correlation between LHβ G1502A polymorphisms in exon 3 of the LH gene and ovarian response to COH. Materials and Methods: A total of 220 women treated with a long protocol for ovarian stimulation were studied. Three genotypes of GG, GA and AA were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: In total, 34 (17%) patients were poor responders, 154 (77%) were normal responders and 12 (6%) were hyper responders. The most frequent genotype was GA (55.5%) whereas 44.5% of patients showed GG genotype and there was no patient with AA genotype. In total 54.5% of normal responders, 61.8% of poor responders and 50% of hyper responders showed GA genotype. Conclusion: Our results did not establish a significant relationship between this polymorphism and the ovarian response. Therefore it is still very difficult to use the genotype of patients for prediction of the ovarian response to stimulation.
format Online
Article
Text
id pubmed-4248152
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Research and Clinical Center for Infertility
record_format MEDLINE/PubMed
spelling pubmed-42481522014-12-02 The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation Davar, Robab Tabibnejad, Nasim Kalantar, Seyed Mehdi Sheikhha, Mohammad Hasan Iran J Reprod Med Original Article Background: Despite extensive progress in IVF techniques, one of the most difficult problems is the variability in the response to controlled ovarian hyperstimulation (COH). Recent studies show the effects of individual genetic variability on COH outcome. Objective: To evaluate the correlation between LHβ G1502A polymorphisms in exon 3 of the LH gene and ovarian response to COH. Materials and Methods: A total of 220 women treated with a long protocol for ovarian stimulation were studied. Three genotypes of GG, GA and AA were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: In total, 34 (17%) patients were poor responders, 154 (77%) were normal responders and 12 (6%) were hyper responders. The most frequent genotype was GA (55.5%) whereas 44.5% of patients showed GG genotype and there was no patient with AA genotype. In total 54.5% of normal responders, 61.8% of poor responders and 50% of hyper responders showed GA genotype. Conclusion: Our results did not establish a significant relationship between this polymorphism and the ovarian response. Therefore it is still very difficult to use the genotype of patients for prediction of the ovarian response to stimulation. Research and Clinical Center for Infertility 2014-10 /pmc/articles/PMC4248152/ /pubmed/25469124 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Davar, Robab
Tabibnejad, Nasim
Kalantar, Seyed Mehdi
Sheikhha, Mohammad Hasan
The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
title The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
title_full The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
title_fullStr The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
title_full_unstemmed The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
title_short The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
title_sort luteinizing hormone beta-subunit exon 3 (gly102ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248152/
https://www.ncbi.nlm.nih.gov/pubmed/25469124
work_keys_str_mv AT davarrobab theluteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT tabibnejadnasim theluteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT kalantarseyedmehdi theluteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT sheikhhamohammadhasan theluteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT davarrobab luteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT tabibnejadnasim luteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT kalantarseyedmehdi luteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation
AT sheikhhamohammadhasan luteinizinghormonebetasubunitexon3gly102sergenemutationandovarianresponsestocontrolledovarianhyperstimulation