Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor

The earliest molecular events in T cell recognition have not yet been fully described, and the initial T cell receptor (TCR) triggering mechanism remains a subject of controversy. Here, using TIRF/FRET microscopy, we observe a two-stage interaction between TCR, CD8, and MHCp. There is an early (with...

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Autores principales: Casas, Javier, Brzostek, Joanna, Zarnitsyna, Veronika I., Hong, Jin-sung, Wei, Qianru, Hoerter, John A.H., Fu, Guo, Ampudia, Jeanette, Zamoyska, Rose, Zhu, Cheng, Gascoigne, Nicholas R.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248239/
https://www.ncbi.nlm.nih.gov/pubmed/25427562
http://dx.doi.org/10.1038/ncomms6624
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author Casas, Javier
Brzostek, Joanna
Zarnitsyna, Veronika I.
Hong, Jin-sung
Wei, Qianru
Hoerter, John A.H.
Fu, Guo
Ampudia, Jeanette
Zamoyska, Rose
Zhu, Cheng
Gascoigne, Nicholas R.J.
author_facet Casas, Javier
Brzostek, Joanna
Zarnitsyna, Veronika I.
Hong, Jin-sung
Wei, Qianru
Hoerter, John A.H.
Fu, Guo
Ampudia, Jeanette
Zamoyska, Rose
Zhu, Cheng
Gascoigne, Nicholas R.J.
author_sort Casas, Javier
collection PubMed
description The earliest molecular events in T cell recognition have not yet been fully described, and the initial T cell receptor (TCR) triggering mechanism remains a subject of controversy. Here, using TIRF/FRET microscopy, we observe a two-stage interaction between TCR, CD8, and MHCp. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR triggering event is induced by free Lck.
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spelling pubmed-42482392015-05-27 Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor Casas, Javier Brzostek, Joanna Zarnitsyna, Veronika I. Hong, Jin-sung Wei, Qianru Hoerter, John A.H. Fu, Guo Ampudia, Jeanette Zamoyska, Rose Zhu, Cheng Gascoigne, Nicholas R.J. Nat Commun Article The earliest molecular events in T cell recognition have not yet been fully described, and the initial T cell receptor (TCR) triggering mechanism remains a subject of controversy. Here, using TIRF/FRET microscopy, we observe a two-stage interaction between TCR, CD8, and MHCp. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR triggering event is induced by free Lck. 2014-11-27 /pmc/articles/PMC4248239/ /pubmed/25427562 http://dx.doi.org/10.1038/ncomms6624 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Casas, Javier
Brzostek, Joanna
Zarnitsyna, Veronika I.
Hong, Jin-sung
Wei, Qianru
Hoerter, John A.H.
Fu, Guo
Ampudia, Jeanette
Zamoyska, Rose
Zhu, Cheng
Gascoigne, Nicholas R.J.
Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor
title Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor
title_full Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor
title_fullStr Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor
title_full_unstemmed Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor
title_short Ligand engaged TCR is triggered by Lck not associated with CD8 coreceptor
title_sort ligand engaged tcr is triggered by lck not associated with cd8 coreceptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248239/
https://www.ncbi.nlm.nih.gov/pubmed/25427562
http://dx.doi.org/10.1038/ncomms6624
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