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A novel method for synthetic vaccine construction based on protein assembly

In the history of vaccine development, the synthetic vaccine is a milestone that is in stark contrast with traditional vaccines based on live-attenuated or inactivated microorganisms. Synthetic vaccines not only are safer than attenuated or inactivated microorganisms but also provide the opportunity...

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Autores principales: Liu, Zhida, Zhou, Hang, Wang, Wenjun, Tan, Wenjie, Fu, Yang-Xin, Zhu, Mingzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248271/
https://www.ncbi.nlm.nih.gov/pubmed/25434527
http://dx.doi.org/10.1038/srep07266
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author Liu, Zhida
Zhou, Hang
Wang, Wenjun
Tan, Wenjie
Fu, Yang-Xin
Zhu, Mingzhao
author_facet Liu, Zhida
Zhou, Hang
Wang, Wenjun
Tan, Wenjie
Fu, Yang-Xin
Zhu, Mingzhao
author_sort Liu, Zhida
collection PubMed
description In the history of vaccine development, the synthetic vaccine is a milestone that is in stark contrast with traditional vaccines based on live-attenuated or inactivated microorganisms. Synthetic vaccines not only are safer than attenuated or inactivated microorganisms but also provide the opportunity for vaccine design for specific purposes. The first generation of synthetic vaccines has been largely based on DNA recombination technology and genetic manipulation. This de novo generation is occasionally time consuming and costly, especially in the era of genomics and when facing pandemic outbreaks of infectious diseases. To accelerate and simplify the R&D process for vaccines, we developed an improved method of synthetic vaccine construction based on protein assembly. We optimized and employed the recently developed SpyTag/SpyCatcher technique to establish a protein assembly system for vaccine generation from pre-prepared subunit proteins. As proof of principle, we chose a dendritic cell (DC)-targeting molecule and specific model antigens to generate desired vaccines. The results demonstrated that a new vaccine generated in this way does not hamper the individual function of different vaccine components and is efficient in inducing both T and B cell responses. This protein assembly strategy may be especially useful for high-throughput antigen screening or rapid vaccine generation.
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spelling pubmed-42482712014-12-08 A novel method for synthetic vaccine construction based on protein assembly Liu, Zhida Zhou, Hang Wang, Wenjun Tan, Wenjie Fu, Yang-Xin Zhu, Mingzhao Sci Rep Article In the history of vaccine development, the synthetic vaccine is a milestone that is in stark contrast with traditional vaccines based on live-attenuated or inactivated microorganisms. Synthetic vaccines not only are safer than attenuated or inactivated microorganisms but also provide the opportunity for vaccine design for specific purposes. The first generation of synthetic vaccines has been largely based on DNA recombination technology and genetic manipulation. This de novo generation is occasionally time consuming and costly, especially in the era of genomics and when facing pandemic outbreaks of infectious diseases. To accelerate and simplify the R&D process for vaccines, we developed an improved method of synthetic vaccine construction based on protein assembly. We optimized and employed the recently developed SpyTag/SpyCatcher technique to establish a protein assembly system for vaccine generation from pre-prepared subunit proteins. As proof of principle, we chose a dendritic cell (DC)-targeting molecule and specific model antigens to generate desired vaccines. The results demonstrated that a new vaccine generated in this way does not hamper the individual function of different vaccine components and is efficient in inducing both T and B cell responses. This protein assembly strategy may be especially useful for high-throughput antigen screening or rapid vaccine generation. Nature Publishing Group 2014-12-01 /pmc/articles/PMC4248271/ /pubmed/25434527 http://dx.doi.org/10.1038/srep07266 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Liu, Zhida
Zhou, Hang
Wang, Wenjun
Tan, Wenjie
Fu, Yang-Xin
Zhu, Mingzhao
A novel method for synthetic vaccine construction based on protein assembly
title A novel method for synthetic vaccine construction based on protein assembly
title_full A novel method for synthetic vaccine construction based on protein assembly
title_fullStr A novel method for synthetic vaccine construction based on protein assembly
title_full_unstemmed A novel method for synthetic vaccine construction based on protein assembly
title_short A novel method for synthetic vaccine construction based on protein assembly
title_sort novel method for synthetic vaccine construction based on protein assembly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248271/
https://www.ncbi.nlm.nih.gov/pubmed/25434527
http://dx.doi.org/10.1038/srep07266
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