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Tn5 transposase and tagmentation procedures for massively scaled sequencing projects

Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing li...

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Detalles Bibliográficos
Autores principales: Picelli, Simone, Björklund, Åsa K., Reinius, Björn, Sagasser, Sven, Winberg, Gösta, Sandberg, Rickard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248319/
https://www.ncbi.nlm.nih.gov/pubmed/25079858
http://dx.doi.org/10.1101/gr.177881.114

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