Characterization of an Acidic Chitinase from Seeds of Black Soybean (Glycine max (L) Merr Tainan No. 3)

Using 4-methylumbelliferyl-β-D-N,N′,N″-triacetylchitotrioside (4-MU-GlcNAc(3)) as a substrate, an acidic chitinase was purified from seeds of black soybean (Glycine max Tainan no. 3) by ammonium sulfate fractionation and three successive steps of column chromatography. The purified chitinase was a m...

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Detalles Bibliográficos
Autores principales: Chang, Ya-Min, Chen, Li-Chun, Wang, Hsin-Yi, Chiang, Chui-Liang, Chang, Chen-Tien, Chung, Yun-Chin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249961/
https://www.ncbi.nlm.nih.gov/pubmed/25437446
http://dx.doi.org/10.1371/journal.pone.0113596

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