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Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock

Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline...

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Autores principales: Amaral, Nathalia Oda, Naves, Lara Marques, Ferreira-Neto, Marcos Luiz, Freiria-Oliveira, André Henrique, Colombari, Eduardo, Rosa, Daniel Alves, Reis, Angela Adamski da Silva, Ianzer, Danielle, Xavier, Carlos Henrique, Pedrino, Gustavo Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251084/
https://www.ncbi.nlm.nih.gov/pubmed/25485300
http://dx.doi.org/10.1155/2014/496121
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author Amaral, Nathalia Oda
Naves, Lara Marques
Ferreira-Neto, Marcos Luiz
Freiria-Oliveira, André Henrique
Colombari, Eduardo
Rosa, Daniel Alves
Reis, Angela Adamski da Silva
Ianzer, Danielle
Xavier, Carlos Henrique
Pedrino, Gustavo Rodrigues
author_facet Amaral, Nathalia Oda
Naves, Lara Marques
Ferreira-Neto, Marcos Luiz
Freiria-Oliveira, André Henrique
Colombari, Eduardo
Rosa, Daniel Alves
Reis, Angela Adamski da Silva
Ianzer, Danielle
Xavier, Carlos Henrique
Pedrino, Gustavo Rodrigues
author_sort Amaral, Nathalia Oda
collection PubMed
description Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABA(A) agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62 ± 1.1 mmHg) and EXP (61 ± 0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104 ± 4.2 versus EXP: 60 ± 6.2 mmHg) and RVC (CONT: 6.4 ± 11.4 versus EXP: -53.5 ± 10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock.
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spelling pubmed-42510842014-12-07 Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock Amaral, Nathalia Oda Naves, Lara Marques Ferreira-Neto, Marcos Luiz Freiria-Oliveira, André Henrique Colombari, Eduardo Rosa, Daniel Alves Reis, Angela Adamski da Silva Ianzer, Danielle Xavier, Carlos Henrique Pedrino, Gustavo Rodrigues ScientificWorldJournal Research Article Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABA(A) agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62 ± 1.1 mmHg) and EXP (61 ± 0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104 ± 4.2 versus EXP: 60 ± 6.2 mmHg) and RVC (CONT: 6.4 ± 11.4 versus EXP: -53.5 ± 10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock. Hindawi Publishing Corporation 2014 2014-11-18 /pmc/articles/PMC4251084/ /pubmed/25485300 http://dx.doi.org/10.1155/2014/496121 Text en Copyright © 2014 Nathalia Oda Amaral et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Amaral, Nathalia Oda
Naves, Lara Marques
Ferreira-Neto, Marcos Luiz
Freiria-Oliveira, André Henrique
Colombari, Eduardo
Rosa, Daniel Alves
Reis, Angela Adamski da Silva
Ianzer, Danielle
Xavier, Carlos Henrique
Pedrino, Gustavo Rodrigues
Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
title Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
title_full Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
title_fullStr Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
title_full_unstemmed Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
title_short Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
title_sort median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251084/
https://www.ncbi.nlm.nih.gov/pubmed/25485300
http://dx.doi.org/10.1155/2014/496121
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