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Membrane domain formation—a key factor for targeted intracellular drug delivery
Protein molecules, toxins and viruses internalize into the cell via receptor-mediated endocytosis (RME) using specific proteins and lipids in the plasma membrane. The plasma membrane is a barrier for many pharmaceutical agents to enter into the cytoplasm of target cells. In the case of cancer cells,...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251288/ https://www.ncbi.nlm.nih.gov/pubmed/25520666 http://dx.doi.org/10.3389/fphys.2014.00462 |
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author | Popov-Čeleketić, Dušan van Bergen en Henegouwen, Paul M. P. |
author_facet | Popov-Čeleketić, Dušan van Bergen en Henegouwen, Paul M. P. |
author_sort | Popov-Čeleketić, Dušan |
collection | PubMed |
description | Protein molecules, toxins and viruses internalize into the cell via receptor-mediated endocytosis (RME) using specific proteins and lipids in the plasma membrane. The plasma membrane is a barrier for many pharmaceutical agents to enter into the cytoplasm of target cells. In the case of cancer cells, tissue-specific biomarkers in the plasma membrane, like cancer-specific growth factor receptors, could be excellent candidates for RME-dependent drug delivery. Recent data suggest that agent binding to these receptors at the cell surface, resulting in membrane domain formation by receptor clustering, can be used for the initiation of RME. As a result, these pharmaceutical agents are internalized into the cells and follow different routes until they reach their final intracellular targets like lysosomes or Golgi. We propose that clustering induced formation of plasma membrane microdomains enriched in receptors, sphingolipids, and inositol lipids, leads to membrane bending which functions as the onset of RME. In this review we will focus on the role of domain formation in RME and discuss potential applications for targeted intracellular drug delivery. |
format | Online Article Text |
id | pubmed-4251288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42512882014-12-17 Membrane domain formation—a key factor for targeted intracellular drug delivery Popov-Čeleketić, Dušan van Bergen en Henegouwen, Paul M. P. Front Physiol Physiology Protein molecules, toxins and viruses internalize into the cell via receptor-mediated endocytosis (RME) using specific proteins and lipids in the plasma membrane. The plasma membrane is a barrier for many pharmaceutical agents to enter into the cytoplasm of target cells. In the case of cancer cells, tissue-specific biomarkers in the plasma membrane, like cancer-specific growth factor receptors, could be excellent candidates for RME-dependent drug delivery. Recent data suggest that agent binding to these receptors at the cell surface, resulting in membrane domain formation by receptor clustering, can be used for the initiation of RME. As a result, these pharmaceutical agents are internalized into the cells and follow different routes until they reach their final intracellular targets like lysosomes or Golgi. We propose that clustering induced formation of plasma membrane microdomains enriched in receptors, sphingolipids, and inositol lipids, leads to membrane bending which functions as the onset of RME. In this review we will focus on the role of domain formation in RME and discuss potential applications for targeted intracellular drug delivery. Frontiers Media S.A. 2014-12-02 /pmc/articles/PMC4251288/ /pubmed/25520666 http://dx.doi.org/10.3389/fphys.2014.00462 Text en Copyright © 2014 Popov-Čeleketić and van Bergen en Henegouwen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Popov-Čeleketić, Dušan van Bergen en Henegouwen, Paul M. P. Membrane domain formation—a key factor for targeted intracellular drug delivery |
title | Membrane domain formation—a key factor for targeted intracellular drug delivery |
title_full | Membrane domain formation—a key factor for targeted intracellular drug delivery |
title_fullStr | Membrane domain formation—a key factor for targeted intracellular drug delivery |
title_full_unstemmed | Membrane domain formation—a key factor for targeted intracellular drug delivery |
title_short | Membrane domain formation—a key factor for targeted intracellular drug delivery |
title_sort | membrane domain formation—a key factor for targeted intracellular drug delivery |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251288/ https://www.ncbi.nlm.nih.gov/pubmed/25520666 http://dx.doi.org/10.3389/fphys.2014.00462 |
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