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Correlation between Plasma Interleukin-3, the α/β Globin Ratio, and Globin mRNA Stability

Background. Globin chain synthesis (GCS) analysis is used in the diagnosis of thalassemia. However, the wide reference range limits its use as a decisive diagnostic tool. It has been shown that α and β   globin mRNA increase through stimulation of cells by interleukin-3 (IL-3). Therefore, this study...

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Detalles Bibliográficos
Autores principales: Rouhi Dehnabeh, S., Mahdian, R., Ajdary, S., Mostafavi, E., Khatami, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251354/
https://www.ncbi.nlm.nih.gov/pubmed/25478214
http://dx.doi.org/10.1155/2014/640203
Descripción
Sumario:Background. Globin chain synthesis (GCS) analysis is used in the diagnosis of thalassemia. However, the wide reference range limits its use as a decisive diagnostic tool. It has been shown that α and β   globin mRNA increase through stimulation of cells by interleukin-3 (IL-3). Therefore, this study investigates the relationship between plasma IL-3 and the β/α   globin ratio. Methods. Blood samples were collected from 32 healthy participants on two occasions one month apart. GCS analysis, real-time PCR, and ELISA tests were conducted to determine the β/α   globin ratio, globin mRNA expression and stability rate, and IL-3 levels. Results. On the basis of IL-3 levels, the participants were divided in two groups. One group included participants who showed a significant increase in IL-3 as indicated by a significant rise in mean values of α, β, and γ   globin mRNA, α and β   globin, RBC, and hemoglobin. The other group included participants who showed no difference in IL-3 levels with no significant variations in the above-mentioned parameters. Conclusion. The results of this study indicate that IL-3 has an equivalent positive effect on α and β   globin chain synthesis. Therefore, IL-3 levels do not explain the wide reference range of the α/β   globin ratio.