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High-resolution structure of a Shigella type III secretion needle by solid-state NMR and cryo-electron microscopy
We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state NMR (ssNMR) chemical shifts and distance constraints de...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251803/ https://www.ncbi.nlm.nih.gov/pubmed/25264107 http://dx.doi.org/10.1038/ncomms5976 |