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AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping

We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaII) and a next-generation sequencing...

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Detalles Bibliográficos
Autores principales: Xia, Zhiqiang, Zou, Meiling, Zhang, Shengkui, Feng, Binxiao, Wang, Wenquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252907/
https://www.ncbi.nlm.nih.gov/pubmed/25466435
http://dx.doi.org/10.1038/srep07300