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Disinfection of gutta-percha cones using three reagents and their residual effects

AIMS AND OBJECTIVES: The purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and MTAD in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. MATERIALS AND METHODS: G...

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Autores principales: Chandrappa, Mahesh Martur, Mundathodu, Nofal, Srinivasan, Raghu, Nasreen, Farhat, Kavitha, Pamarthi, Shetty, Ashwija
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252933/
https://www.ncbi.nlm.nih.gov/pubmed/25506147
http://dx.doi.org/10.4103/0972-0707.144607
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author Chandrappa, Mahesh Martur
Mundathodu, Nofal
Srinivasan, Raghu
Nasreen, Farhat
Kavitha, Pamarthi
Shetty, Ashwija
author_facet Chandrappa, Mahesh Martur
Mundathodu, Nofal
Srinivasan, Raghu
Nasreen, Farhat
Kavitha, Pamarthi
Shetty, Ashwija
author_sort Chandrappa, Mahesh Martur
collection PubMed
description AIMS AND OBJECTIVES: The purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and MTAD in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. MATERIALS AND METHODS: Gutta-percha cones were immersed in suspensions of Enterococcus faecalis and Staphylococcus aureus separately. The cones were then immersed in 5.25% NaOCl, 2% CHX and MTAD for 30 seconds, 1 minute and 5 minutes separately. The disinfected cones were then incubated in thioglycollate media for 7 days. The thioglycolate media was sub-cultured and colony forming units were counted. For topographical examination of gutta-percha cones, the cones were immersed in respective solutions for one minute and allowed to air dry for 30 minutes after rinsing or without rinsing the cones with distilled water. The topography of the cones were then analyzed under SEM. Data was statistically analyzed using one way anova. RESULTS: MTAD was found to be the most effective disinfecting solution. The topographical examination of GP cones found some deposits after the disinfection procedure in every group. These deposits were removed when the GP cones were rinsed with distilled water. CONCLUSIONS: MTAD possesses superior bactericidal activity when compared with NaOCl and CHX and a final rinse with distilled water is essential after the disinfection procedure.
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spelling pubmed-42529332014-12-12 Disinfection of gutta-percha cones using three reagents and their residual effects Chandrappa, Mahesh Martur Mundathodu, Nofal Srinivasan, Raghu Nasreen, Farhat Kavitha, Pamarthi Shetty, Ashwija J Conserv Dent Original Article AIMS AND OBJECTIVES: The purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and MTAD in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. MATERIALS AND METHODS: Gutta-percha cones were immersed in suspensions of Enterococcus faecalis and Staphylococcus aureus separately. The cones were then immersed in 5.25% NaOCl, 2% CHX and MTAD for 30 seconds, 1 minute and 5 minutes separately. The disinfected cones were then incubated in thioglycollate media for 7 days. The thioglycolate media was sub-cultured and colony forming units were counted. For topographical examination of gutta-percha cones, the cones were immersed in respective solutions for one minute and allowed to air dry for 30 minutes after rinsing or without rinsing the cones with distilled water. The topography of the cones were then analyzed under SEM. Data was statistically analyzed using one way anova. RESULTS: MTAD was found to be the most effective disinfecting solution. The topographical examination of GP cones found some deposits after the disinfection procedure in every group. These deposits were removed when the GP cones were rinsed with distilled water. CONCLUSIONS: MTAD possesses superior bactericidal activity when compared with NaOCl and CHX and a final rinse with distilled water is essential after the disinfection procedure. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4252933/ /pubmed/25506147 http://dx.doi.org/10.4103/0972-0707.144607 Text en Copyright: © Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chandrappa, Mahesh Martur
Mundathodu, Nofal
Srinivasan, Raghu
Nasreen, Farhat
Kavitha, Pamarthi
Shetty, Ashwija
Disinfection of gutta-percha cones using three reagents and their residual effects
title Disinfection of gutta-percha cones using three reagents and their residual effects
title_full Disinfection of gutta-percha cones using three reagents and their residual effects
title_fullStr Disinfection of gutta-percha cones using three reagents and their residual effects
title_full_unstemmed Disinfection of gutta-percha cones using three reagents and their residual effects
title_short Disinfection of gutta-percha cones using three reagents and their residual effects
title_sort disinfection of gutta-percha cones using three reagents and their residual effects
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252933/
https://www.ncbi.nlm.nih.gov/pubmed/25506147
http://dx.doi.org/10.4103/0972-0707.144607
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