Cargando…

An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines

BACKGROUND: The Autotransporter pathway, ubiquitous in Gram-negative bacteria, allows the efficient secretion of large passenger proteins via a relatively simple mechanism. Capitalizing on its crystal structure, we have engineered the Escherichia coli autotransporter Hemoglobin protease (Hbp) into a...

Descripción completa

Detalles Bibliográficos
Autores principales: Jong, Wouter SP, Daleke-Schermerhorn, Maria H, Vikström, David, ten Hagen-Jongman, Corinne M, de Punder, Karin, van der Wel, Nicole N, van de Sandt, Carolien E, Rimmelzwaan, Guus F, Follmann, Frank, Agger, Else Marie, Andersen, Peter, de Gier, Jan-Willem, Luirink, Joen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252983/
https://www.ncbi.nlm.nih.gov/pubmed/25421093
http://dx.doi.org/10.1186/s12934-014-0162-8
_version_ 1782347210793418752
author Jong, Wouter SP
Daleke-Schermerhorn, Maria H
Vikström, David
ten Hagen-Jongman, Corinne M
de Punder, Karin
van der Wel, Nicole N
van de Sandt, Carolien E
Rimmelzwaan, Guus F
Follmann, Frank
Agger, Else Marie
Andersen, Peter
de Gier, Jan-Willem
Luirink, Joen
author_facet Jong, Wouter SP
Daleke-Schermerhorn, Maria H
Vikström, David
ten Hagen-Jongman, Corinne M
de Punder, Karin
van der Wel, Nicole N
van de Sandt, Carolien E
Rimmelzwaan, Guus F
Follmann, Frank
Agger, Else Marie
Andersen, Peter
de Gier, Jan-Willem
Luirink, Joen
author_sort Jong, Wouter SP
collection PubMed
description BACKGROUND: The Autotransporter pathway, ubiquitous in Gram-negative bacteria, allows the efficient secretion of large passenger proteins via a relatively simple mechanism. Capitalizing on its crystal structure, we have engineered the Escherichia coli autotransporter Hemoglobin protease (Hbp) into a versatile platform for secretion and surface display of multiple heterologous proteins in one carrier molecule. RESULTS: As proof-of-concept, we demonstrate efficient secretion and high-density display of the sizeable Mycobacterium tuberculosis antigens ESAT6, Ag85B and Rv2660c in E. coli simultaneously. Furthermore, we show stable multivalent display of these antigens in an attenuated Salmonella Typhimurium strain upon chromosomal integration. To emphasize the versatility of the Hbp platform, we also demonstrate efficient expression of multiple sizeable antigenic fragments from Chlamydia trachomatis and the influenza A virus at the Salmonella cell surface. CONCLUSIONS: The successful efficient cell surface display of multiple antigens from various pathogenic organisms highlights the potential of Hbp as a universal platform for the development of multivalent recombinant bacterial vector vaccines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-014-0162-8) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4252983
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42529832014-12-04 An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines Jong, Wouter SP Daleke-Schermerhorn, Maria H Vikström, David ten Hagen-Jongman, Corinne M de Punder, Karin van der Wel, Nicole N van de Sandt, Carolien E Rimmelzwaan, Guus F Follmann, Frank Agger, Else Marie Andersen, Peter de Gier, Jan-Willem Luirink, Joen Microb Cell Fact Research BACKGROUND: The Autotransporter pathway, ubiquitous in Gram-negative bacteria, allows the efficient secretion of large passenger proteins via a relatively simple mechanism. Capitalizing on its crystal structure, we have engineered the Escherichia coli autotransporter Hemoglobin protease (Hbp) into a versatile platform for secretion and surface display of multiple heterologous proteins in one carrier molecule. RESULTS: As proof-of-concept, we demonstrate efficient secretion and high-density display of the sizeable Mycobacterium tuberculosis antigens ESAT6, Ag85B and Rv2660c in E. coli simultaneously. Furthermore, we show stable multivalent display of these antigens in an attenuated Salmonella Typhimurium strain upon chromosomal integration. To emphasize the versatility of the Hbp platform, we also demonstrate efficient expression of multiple sizeable antigenic fragments from Chlamydia trachomatis and the influenza A virus at the Salmonella cell surface. CONCLUSIONS: The successful efficient cell surface display of multiple antigens from various pathogenic organisms highlights the potential of Hbp as a universal platform for the development of multivalent recombinant bacterial vector vaccines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-014-0162-8) contains supplementary material, which is available to authorized users. BioMed Central 2014-11-25 /pmc/articles/PMC4252983/ /pubmed/25421093 http://dx.doi.org/10.1186/s12934-014-0162-8 Text en © Jong et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Jong, Wouter SP
Daleke-Schermerhorn, Maria H
Vikström, David
ten Hagen-Jongman, Corinne M
de Punder, Karin
van der Wel, Nicole N
van de Sandt, Carolien E
Rimmelzwaan, Guus F
Follmann, Frank
Agger, Else Marie
Andersen, Peter
de Gier, Jan-Willem
Luirink, Joen
An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
title An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
title_full An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
title_fullStr An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
title_full_unstemmed An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
title_short An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
title_sort autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252983/
https://www.ncbi.nlm.nih.gov/pubmed/25421093
http://dx.doi.org/10.1186/s12934-014-0162-8
work_keys_str_mv AT jongwoutersp anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT dalekeschermerhornmariah anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT vikstromdavid anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT tenhagenjongmancorinnem anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT depunderkarin anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT vanderwelnicolen anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT vandesandtcaroliene anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT rimmelzwaanguusf anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT follmannfrank anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT aggerelsemarie anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT andersenpeter anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT degierjanwillem anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT luirinkjoen anautotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT jongwoutersp autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT dalekeschermerhornmariah autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT vikstromdavid autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT tenhagenjongmancorinnem autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT depunderkarin autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT vanderwelnicolen autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT vandesandtcaroliene autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT rimmelzwaanguusf autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT follmannfrank autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT aggerelsemarie autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT andersenpeter autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT degierjanwillem autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines
AT luirinkjoen autotransporterdisplayplatformforthedevelopmentofmultivalentrecombinantbacterialvectorvaccines