Cargando…

What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls

There is evidence of blood-borne miRNA signatures for various human diseases. To dissect the origin of disease-specific miRNA expression in human blood, we separately analyzed the miRNome of different immune cell subtypes, each in lung cancer patients and healthy individuals. Each immune cell type r...

Descripción completa

Detalles Bibliográficos
Autores principales: Leidinger, Petra, Backes, Christina, Dahmke, Indra N., Galata, Valentina, Huwer, Hanno, Stehle, Ingo, Bals, Robert, Keller, Andreas, Meese, Eckart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253448/
https://www.ncbi.nlm.nih.gov/pubmed/25344866
_version_ 1782347260390014976
author Leidinger, Petra
Backes, Christina
Dahmke, Indra N.
Galata, Valentina
Huwer, Hanno
Stehle, Ingo
Bals, Robert
Keller, Andreas
Meese, Eckart
author_facet Leidinger, Petra
Backes, Christina
Dahmke, Indra N.
Galata, Valentina
Huwer, Hanno
Stehle, Ingo
Bals, Robert
Keller, Andreas
Meese, Eckart
author_sort Leidinger, Petra
collection PubMed
description There is evidence of blood-borne miRNA signatures for various human diseases. To dissect the origin of disease-specific miRNA expression in human blood, we separately analyzed the miRNome of different immune cell subtypes, each in lung cancer patients and healthy individuals. Each immune cell type revealed a specific miRNA expression pattern also dependinging on the cell origin, line of defense, and function. The overall expression pattern of each leukocyte subtype showed great similarities between patients and controls. However, for each cell subtype we identified miRNAs that were deregulated in lung cancer patients including hsa-miR-21, a well-known oncomiR associated with poor lung cancer prognosis that was up-regulated in all leukocyte subtype comparisons of cancer versus controls. While the miRNome of cells of the adaptive immune system allowed only a weak separation between patients and controls, cells of the innate immune system allowed perfect or nearly perfect classification. Leukocytes of lung cancer patients show a cancer-specific miRNA expression profile. Our data also show that cancer specific miRNA expression pattern of whole blood samples are not determined by a single cell type. The data indicate that additional blood components, like erythrocytes, platelets, or exosomes might contribute to the disease specificity of a miRNA signature.
format Online
Article
Text
id pubmed-4253448
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-42534482014-12-03 What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls Leidinger, Petra Backes, Christina Dahmke, Indra N. Galata, Valentina Huwer, Hanno Stehle, Ingo Bals, Robert Keller, Andreas Meese, Eckart Oncotarget Research Paper There is evidence of blood-borne miRNA signatures for various human diseases. To dissect the origin of disease-specific miRNA expression in human blood, we separately analyzed the miRNome of different immune cell subtypes, each in lung cancer patients and healthy individuals. Each immune cell type revealed a specific miRNA expression pattern also dependinging on the cell origin, line of defense, and function. The overall expression pattern of each leukocyte subtype showed great similarities between patients and controls. However, for each cell subtype we identified miRNAs that were deregulated in lung cancer patients including hsa-miR-21, a well-known oncomiR associated with poor lung cancer prognosis that was up-regulated in all leukocyte subtype comparisons of cancer versus controls. While the miRNome of cells of the adaptive immune system allowed only a weak separation between patients and controls, cells of the innate immune system allowed perfect or nearly perfect classification. Leukocytes of lung cancer patients show a cancer-specific miRNA expression profile. Our data also show that cancer specific miRNA expression pattern of whole blood samples are not determined by a single cell type. The data indicate that additional blood components, like erythrocytes, platelets, or exosomes might contribute to the disease specificity of a miRNA signature. Impact Journals LLC 2014-09-19 /pmc/articles/PMC4253448/ /pubmed/25344866 Text en Copyright: © 2014 Leidinger et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Leidinger, Petra
Backes, Christina
Dahmke, Indra N.
Galata, Valentina
Huwer, Hanno
Stehle, Ingo
Bals, Robert
Keller, Andreas
Meese, Eckart
What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls
title What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls
title_full What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls
title_fullStr What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls
title_full_unstemmed What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls
title_short What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls
title_sort what makes a blood cell based mirna expression pattern disease specific? - a mirnome analysis of blood cell subsets in lung cancer patients and healthy controls
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253448/
https://www.ncbi.nlm.nih.gov/pubmed/25344866
work_keys_str_mv AT leidingerpetra whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT backeschristina whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT dahmkeindran whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT galatavalentina whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT huwerhanno whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT stehleingo whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT balsrobert whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT kellerandreas whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols
AT meeseeckart whatmakesabloodcellbasedmirnaexpressionpatterndiseasespecificamirnomeanalysisofbloodcellsubsetsinlungcancerpatientsandhealthycontrols