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Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran
Human tuberculosis caused by Mycobacterium tuberculosis (Mtb) remains a significant disease in many countries. According to Iran's borders with Afghanistan and Pakistan, which are among the 22 high burden countries around the world, this study was conducted to analyze the current molecular epid...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253705/ https://www.ncbi.nlm.nih.gov/pubmed/25505987 http://dx.doi.org/10.1155/2014/195287 |
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author | Soleimanpour, Saman Hamedi Asl, Daryoush Tadayon, Keyvan Farazi, Ali Asghar Keshavarz, Rouhollah Soleymani, Kioomars Seddighinia, Fereshteh Sadat Mosavari, Nader |
author_facet | Soleimanpour, Saman Hamedi Asl, Daryoush Tadayon, Keyvan Farazi, Ali Asghar Keshavarz, Rouhollah Soleymani, Kioomars Seddighinia, Fereshteh Sadat Mosavari, Nader |
author_sort | Soleimanpour, Saman |
collection | PubMed |
description | Human tuberculosis caused by Mycobacterium tuberculosis (Mtb) remains a significant disease in many countries. According to Iran's borders with Afghanistan and Pakistan, which are among the 22 high burden countries around the world, this study was conducted to analyze the current molecular epidemiology of tuberculosis and survey genetic diversity of Mtb strains in Markazi Province in center of Iran. In this experimental study, 75 sputum specimens and one gastric lavage from all smear-positive TB patients admitted to the public hospitals across the Markazi Province were cultured on specific mycobacterial culture media. Genomic DNA was digested by PvuII and transferred to positively charged nylon membrane by southern blotting method and hybridization by PGRS and DR probes. Genotyping of the isolates by PGRS-RFLP and DR-RFLP displayed a wide range of genetic diversity as 25 and 26 genotypes were identified, respectively. Generally speaking, despite the relatively limited number of isolates in the study, high age of patients and also large heterogeneity found in the setting are both in opposition to active circulation of Mtb strains between patients under study either Iranian or Afghan nationals. Thus, it seems that reactivation of latent infection has had the main role in the spread of tuberculosis. |
format | Online Article Text |
id | pubmed-4253705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42537052014-12-11 Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran Soleimanpour, Saman Hamedi Asl, Daryoush Tadayon, Keyvan Farazi, Ali Asghar Keshavarz, Rouhollah Soleymani, Kioomars Seddighinia, Fereshteh Sadat Mosavari, Nader Tuberc Res Treat Research Article Human tuberculosis caused by Mycobacterium tuberculosis (Mtb) remains a significant disease in many countries. According to Iran's borders with Afghanistan and Pakistan, which are among the 22 high burden countries around the world, this study was conducted to analyze the current molecular epidemiology of tuberculosis and survey genetic diversity of Mtb strains in Markazi Province in center of Iran. In this experimental study, 75 sputum specimens and one gastric lavage from all smear-positive TB patients admitted to the public hospitals across the Markazi Province were cultured on specific mycobacterial culture media. Genomic DNA was digested by PvuII and transferred to positively charged nylon membrane by southern blotting method and hybridization by PGRS and DR probes. Genotyping of the isolates by PGRS-RFLP and DR-RFLP displayed a wide range of genetic diversity as 25 and 26 genotypes were identified, respectively. Generally speaking, despite the relatively limited number of isolates in the study, high age of patients and also large heterogeneity found in the setting are both in opposition to active circulation of Mtb strains between patients under study either Iranian or Afghan nationals. Thus, it seems that reactivation of latent infection has had the main role in the spread of tuberculosis. Hindawi Publishing Corporation 2014 2014-11-19 /pmc/articles/PMC4253705/ /pubmed/25505987 http://dx.doi.org/10.1155/2014/195287 Text en Copyright © 2014 Saman Soleimanpour et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Soleimanpour, Saman Hamedi Asl, Daryoush Tadayon, Keyvan Farazi, Ali Asghar Keshavarz, Rouhollah Soleymani, Kioomars Seddighinia, Fereshteh Sadat Mosavari, Nader Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran |
title | Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran |
title_full | Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran |
title_fullStr | Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran |
title_full_unstemmed | Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran |
title_short | Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran |
title_sort | extensive genetic diversity among clinical isolates of mycobacterium tuberculosis in central province of iran |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253705/ https://www.ncbi.nlm.nih.gov/pubmed/25505987 http://dx.doi.org/10.1155/2014/195287 |
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