Tel1(ATM) dictates the replication timing of short yeast telomeres

Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double-strand breaks flanked by short telomeric arrays cause origin firing early in S pha...

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Detalles Bibliográficos
Autores principales: Cooley, Carol, Davé, Anoushka, Garg, Mansi, Bianchi, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Scientific Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253850/
https://www.ncbi.nlm.nih.gov/pubmed/25122631
http://dx.doi.org/10.15252/embr.201439242
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author Cooley, Carol
Davé, Anoushka
Garg, Mansi
Bianchi, Alessandro
author_facet Cooley, Carol
Davé, Anoushka
Garg, Mansi
Bianchi, Alessandro
author_sort Cooley, Carol
collection PubMed
description Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double-strand breaks flanked by short telomeric arrays cause origin firing early in S phase at late-replicating loci and that this effect on origin firing time is dependent on the Tel1(ATM) checkpoint kinase. The effect of Tel1(ATM) on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss. These results establish that Tel1(ATM) specifies not only the extent but also the timing of telomerase recruitment.
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spelling pubmed-42538502015-10-01 Tel1(ATM) dictates the replication timing of short yeast telomeres Cooley, Carol Davé, Anoushka Garg, Mansi Bianchi, Alessandro EMBO Rep Scientific Reports Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double-strand breaks flanked by short telomeric arrays cause origin firing early in S phase at late-replicating loci and that this effect on origin firing time is dependent on the Tel1(ATM) checkpoint kinase. The effect of Tel1(ATM) on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss. These results establish that Tel1(ATM) specifies not only the extent but also the timing of telomerase recruitment. Blackwell Publishing Ltd 2014-10 2014-08-13 /pmc/articles/PMC4253850/ /pubmed/25122631 http://dx.doi.org/10.15252/embr.201439242 Text en © 2014 The Authors. Published under the terms of the CC BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Scientific Reports
Cooley, Carol
Davé, Anoushka
Garg, Mansi
Bianchi, Alessandro
Tel1(ATM) dictates the replication timing of short yeast telomeres
title Tel1(ATM) dictates the replication timing of short yeast telomeres
title_full Tel1(ATM) dictates the replication timing of short yeast telomeres
title_fullStr Tel1(ATM) dictates the replication timing of short yeast telomeres
title_full_unstemmed Tel1(ATM) dictates the replication timing of short yeast telomeres
title_short Tel1(ATM) dictates the replication timing of short yeast telomeres
title_sort tel1(atm) dictates the replication timing of short yeast telomeres
topic Scientific Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253850/
https://www.ncbi.nlm.nih.gov/pubmed/25122631
http://dx.doi.org/10.15252/embr.201439242
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