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Comparison of Whole Blood RNA Preservation Tubes and Novel Generation RNA Extraction Kits for Analysis of mRNA and MiRNA Profiles
BACKGROUND: Whole blood expression profiling is frequently performed using PAXgene (Qiagen) or Tempus (Life Technologies) tubes. Here, we compare 6 novel generation RNA isolation protocols with respect to RNA quantity, quality and recovery of mRNA and miRNA. METHODS: 3 PAXgene and 3 Tempus Tubes wer...
Autores principales: | Häntzsch, Madlen, Tolios, Alexander, Beutner, Frank, Nagel, Dorothea, Thiery, Joachim, Teupser, Daniel, Holdt, Lesca M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4254602/ https://www.ncbi.nlm.nih.gov/pubmed/25469788 http://dx.doi.org/10.1371/journal.pone.0113298 |
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