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The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles

Inherent nanomaterial characteristics, composition, surface chemistry, and primary particle size, are known to impact particle stability, uptake, and toxicity. Nanocomposites challenge our ability to predict nanoparticle reactivity in biological systems if they are composed of materials with contras...

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Detalles Bibliográficos
Autores principales: Bonventre, Josephine A., Pryor, Joseph B., Harper, Bryan J., Harper, Stacey L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255064/
https://www.ncbi.nlm.nih.gov/pubmed/25484618
http://dx.doi.org/10.1007/s11051-014-2761-z
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author Bonventre, Josephine A.
Pryor, Joseph B.
Harper, Bryan J.
Harper, Stacey L.
author_facet Bonventre, Josephine A.
Pryor, Joseph B.
Harper, Bryan J.
Harper, Stacey L.
author_sort Bonventre, Josephine A.
collection PubMed
description Inherent nanomaterial characteristics, composition, surface chemistry, and primary particle size, are known to impact particle stability, uptake, and toxicity. Nanocomposites challenge our ability to predict nanoparticle reactivity in biological systems if they are composed of materials with contrasting relative toxicities. We hypothesized that toxicity would be dominated by the nanoparticle surface (shell vs core), and that modulating the surface ligands would have a direct impact on uptake. We exposed developing zebrafish (Danio rerio) to a series of ~70 nm amine-terminated silver nanoparticles with silica shells (AgSi NPs) to investigate the relative influence of surface amination, composition, and size on toxicity. Like-sized aminated AgSi and Si NPs were more toxic than paired hydroxyl-terminated nanoparticles; however, both AgSi NPs were more toxic than the Si NPs, indicating a significant contribution of the silver core to the toxicity. Incremental increases in surface amination did not linearly increase uptake and toxicity, but did have a marked impact on dispersion stability. Mass-based exposure metrics initially supported the hypothesis that smaller nanoparticles (20 nm) would be more toxic than larger particles (70 nm). However, surface area-based metrics revealed that toxicity was independent of size. Our studies suggest that nanoparticle surfaces play a critical role in the uptake and toxicity of AgSi NPs, while the impact of size may be a function of the exposure metric used. Overall, uptake and toxicity can be dramatically altered by small changes in surface functionalization or exposure media. Only after understanding the magnitude of these changes, can we begin to understand the biologically available dose following nanoparticle exposure. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11051-014-2761-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-42550642014-12-05 The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles Bonventre, Josephine A. Pryor, Joseph B. Harper, Bryan J. Harper, Stacey L. J Nanopart Res Research Paper Inherent nanomaterial characteristics, composition, surface chemistry, and primary particle size, are known to impact particle stability, uptake, and toxicity. Nanocomposites challenge our ability to predict nanoparticle reactivity in biological systems if they are composed of materials with contrasting relative toxicities. We hypothesized that toxicity would be dominated by the nanoparticle surface (shell vs core), and that modulating the surface ligands would have a direct impact on uptake. We exposed developing zebrafish (Danio rerio) to a series of ~70 nm amine-terminated silver nanoparticles with silica shells (AgSi NPs) to investigate the relative influence of surface amination, composition, and size on toxicity. Like-sized aminated AgSi and Si NPs were more toxic than paired hydroxyl-terminated nanoparticles; however, both AgSi NPs were more toxic than the Si NPs, indicating a significant contribution of the silver core to the toxicity. Incremental increases in surface amination did not linearly increase uptake and toxicity, but did have a marked impact on dispersion stability. Mass-based exposure metrics initially supported the hypothesis that smaller nanoparticles (20 nm) would be more toxic than larger particles (70 nm). However, surface area-based metrics revealed that toxicity was independent of size. Our studies suggest that nanoparticle surfaces play a critical role in the uptake and toxicity of AgSi NPs, while the impact of size may be a function of the exposure metric used. Overall, uptake and toxicity can be dramatically altered by small changes in surface functionalization or exposure media. Only after understanding the magnitude of these changes, can we begin to understand the biologically available dose following nanoparticle exposure. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11051-014-2761-z) contains supplementary material, which is available to authorized users. Springer Netherlands 2014-12-04 2014 /pmc/articles/PMC4255064/ /pubmed/25484618 http://dx.doi.org/10.1007/s11051-014-2761-z Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research Paper
Bonventre, Josephine A.
Pryor, Joseph B.
Harper, Bryan J.
Harper, Stacey L.
The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
title The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
title_full The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
title_fullStr The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
title_full_unstemmed The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
title_short The impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
title_sort impact of aminated surface ligands and silica shells on the stability, uptake, and toxicity of engineered silver nanoparticles
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255064/
https://www.ncbi.nlm.nih.gov/pubmed/25484618
http://dx.doi.org/10.1007/s11051-014-2761-z
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