Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes

The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis...

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Detalles Bibliográficos
Autores principales: Dai, Ping, Harada, Yoshinori, Miyachi, Hitoshi, Tanaka, Hideo, Kitano, Satsuki, Adachi, Tetsuya, Suzuki, Tomoyuki, Hino, Hitoshi, Takamatsu, Tetsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255192/
https://www.ncbi.nlm.nih.gov/pubmed/25471520
http://dx.doi.org/10.1038/srep07323
Descripción
Sumario:The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis in the target cells would be a potential risk. Here we report reprogramming of siPSCs (gene silencing-induced pluripotent stem cells) from mouse neonatal cardiomyocytes (CMs) by combining TGF-β signal inhibition and connexin43 (Cx43) silencing, and show that siPSCs show pluripotency in vitro and in vivo. Our novel non-insertional mutagenesis technique may provide a means for iPSC generation.

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