Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes

The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis...

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Autores principales: Dai, Ping, Harada, Yoshinori, Miyachi, Hitoshi, Tanaka, Hideo, Kitano, Satsuki, Adachi, Tetsuya, Suzuki, Tomoyuki, Hino, Hitoshi, Takamatsu, Tetsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255192/
https://www.ncbi.nlm.nih.gov/pubmed/25471520
http://dx.doi.org/10.1038/srep07323
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author Dai, Ping
Harada, Yoshinori
Miyachi, Hitoshi
Tanaka, Hideo
Kitano, Satsuki
Adachi, Tetsuya
Suzuki, Tomoyuki
Hino, Hitoshi
Takamatsu, Tetsuro
author_facet Dai, Ping
Harada, Yoshinori
Miyachi, Hitoshi
Tanaka, Hideo
Kitano, Satsuki
Adachi, Tetsuya
Suzuki, Tomoyuki
Hino, Hitoshi
Takamatsu, Tetsuro
author_sort Dai, Ping
collection PubMed
description The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis in the target cells would be a potential risk. Here we report reprogramming of siPSCs (gene silencing-induced pluripotent stem cells) from mouse neonatal cardiomyocytes (CMs) by combining TGF-β signal inhibition and connexin43 (Cx43) silencing, and show that siPSCs show pluripotency in vitro and in vivo. Our novel non-insertional mutagenesis technique may provide a means for iPSC generation.
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spelling pubmed-42551922014-12-08 Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes Dai, Ping Harada, Yoshinori Miyachi, Hitoshi Tanaka, Hideo Kitano, Satsuki Adachi, Tetsuya Suzuki, Tomoyuki Hino, Hitoshi Takamatsu, Tetsuro Sci Rep Article The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis in the target cells would be a potential risk. Here we report reprogramming of siPSCs (gene silencing-induced pluripotent stem cells) from mouse neonatal cardiomyocytes (CMs) by combining TGF-β signal inhibition and connexin43 (Cx43) silencing, and show that siPSCs show pluripotency in vitro and in vivo. Our novel non-insertional mutagenesis technique may provide a means for iPSC generation. Nature Publishing Group 2014-12-04 /pmc/articles/PMC4255192/ /pubmed/25471520 http://dx.doi.org/10.1038/srep07323 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Dai, Ping
Harada, Yoshinori
Miyachi, Hitoshi
Tanaka, Hideo
Kitano, Satsuki
Adachi, Tetsuya
Suzuki, Tomoyuki
Hino, Hitoshi
Takamatsu, Tetsuro
Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes
title Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes
title_full Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes
title_fullStr Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes
title_full_unstemmed Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes
title_short Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes
title_sort combining tgf-β signal inhibition and connexin43 silencing for ipsc induction from mouse cardiomyocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255192/
https://www.ncbi.nlm.nih.gov/pubmed/25471520
http://dx.doi.org/10.1038/srep07323
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