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Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics

This study examines how differentiation of human mesenchymal stem cells regulates the interaction between the cell membrane and the actin cortex controlling cell behavior. Micropipette aspiration was used to measure the pressure required for membrane-cortex detachment which increased from 0.15 kPa i...

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Autores principales: Sliogeryte, Kristina, Thorpe, Stephen D., Lee, David A., Botto, Lorenzo, Knight, Martin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255193/
https://www.ncbi.nlm.nih.gov/pubmed/25471686
http://dx.doi.org/10.1038/srep07307
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author Sliogeryte, Kristina
Thorpe, Stephen D.
Lee, David A.
Botto, Lorenzo
Knight, Martin M.
author_facet Sliogeryte, Kristina
Thorpe, Stephen D.
Lee, David A.
Botto, Lorenzo
Knight, Martin M.
author_sort Sliogeryte, Kristina
collection PubMed
description This study examines how differentiation of human mesenchymal stem cells regulates the interaction between the cell membrane and the actin cortex controlling cell behavior. Micropipette aspiration was used to measure the pressure required for membrane-cortex detachment which increased from 0.15 kPa in stem cells to 0.71 kPa following chondrogenic differentiation. This effect was associated with reduced susceptibility to mechanical and osmotic bleb formation, reduced migration and an increase in cell modulus. Theoretical modelling of bleb formation demonstrated that the increased stiffness of differentiated cells was due to the increased membrane-cortex adhesion. Differentiated cells exhibited greater F-actin density and slower actin remodelling. Differentiated cells also expressed greater levels of the membrane-cortex ezrin, radixin, moeisin (ERM) linker proteins which was responsible for the reduced blebability, as confirmed by transfection of stem cells with dominant active ezrin-T567D-GFP. This study demonstrates that stem cells have an inherently weak membrane-cortex adhesion which increases blebability thereby regulating cell migration and stiffness.
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spelling pubmed-42551932014-12-08 Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics Sliogeryte, Kristina Thorpe, Stephen D. Lee, David A. Botto, Lorenzo Knight, Martin M. Sci Rep Article This study examines how differentiation of human mesenchymal stem cells regulates the interaction between the cell membrane and the actin cortex controlling cell behavior. Micropipette aspiration was used to measure the pressure required for membrane-cortex detachment which increased from 0.15 kPa in stem cells to 0.71 kPa following chondrogenic differentiation. This effect was associated with reduced susceptibility to mechanical and osmotic bleb formation, reduced migration and an increase in cell modulus. Theoretical modelling of bleb formation demonstrated that the increased stiffness of differentiated cells was due to the increased membrane-cortex adhesion. Differentiated cells exhibited greater F-actin density and slower actin remodelling. Differentiated cells also expressed greater levels of the membrane-cortex ezrin, radixin, moeisin (ERM) linker proteins which was responsible for the reduced blebability, as confirmed by transfection of stem cells with dominant active ezrin-T567D-GFP. This study demonstrates that stem cells have an inherently weak membrane-cortex adhesion which increases blebability thereby regulating cell migration and stiffness. Nature Publishing Group 2014-12-04 /pmc/articles/PMC4255193/ /pubmed/25471686 http://dx.doi.org/10.1038/srep07307 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sliogeryte, Kristina
Thorpe, Stephen D.
Lee, David A.
Botto, Lorenzo
Knight, Martin M.
Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
title Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
title_full Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
title_fullStr Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
title_full_unstemmed Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
title_short Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
title_sort stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255193/
https://www.ncbi.nlm.nih.gov/pubmed/25471686
http://dx.doi.org/10.1038/srep07307
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