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Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis

BACKGROUND: Since adenoids may act as a reservoir for bacteria, they can cause ear infection, recurrent otitis and recurrent adenotonsillitis. Therefore, adenotonsillectomy is an efficient method for reducing the number and severity of subsequent infections. OBJECTIVES: This study aimed to determine...

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Autores principales: Javadi Nia, Shima, Zarabi, Vida, Noorbakhsh, Samileh, Farhadi, Mohammad, Ghavidel Darestani, Sahar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255211/
https://www.ncbi.nlm.nih.gov/pubmed/25485049
http://dx.doi.org/10.5812/jjm.11134
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author Javadi Nia, Shima
Zarabi, Vida
Noorbakhsh, Samileh
Farhadi, Mohammad
Ghavidel Darestani, Sahar
author_facet Javadi Nia, Shima
Zarabi, Vida
Noorbakhsh, Samileh
Farhadi, Mohammad
Ghavidel Darestani, Sahar
author_sort Javadi Nia, Shima
collection PubMed
description BACKGROUND: Since adenoids may act as a reservoir for bacteria, they can cause ear infection, recurrent otitis and recurrent adenotonsillitis. Therefore, adenotonsillectomy is an efficient method for reducing the number and severity of subsequent infections. OBJECTIVES: This study aimed to determine the Chlamydophila pneumonia infection by serological tests and performing PCR in the adenoid tissue, in children undergoing adenoid surgery and compare the results with normal children. PATIENTS AND METHODS: This case control study was conducted in the ENT and pediatric wards of Rasoul Akram Hospital in Tehran (2008-2011). We studied 53 patients who had undergone the adenoid surgery (adenoid hypertrophy concomitant with rhino sinusitis) and 31 other patients undergoing elective surgeries in the general surgery ward (like orthopedic surgeries, hernia, etc.) without any infection (like rhinosinusitis), as the control groups. We searched for C. pneumoniae by PCR kits (Chemicon, Germany) in resected adenoid tissues and nasopharyngeal swabs of controls (ethics restrictions in controls). We also looked for specific IgM & IgG antibodies (ELISA, Biochem Immuno Systems, Italy) and compared them between the two groups. A P value < 0.05 was considered statistically significant. RESULTS: Positive PCR results were observed in 13.5% of cases and none of the controls. The condition prevalence was higher in warm seasons (P = 0.05). No correlation was found between positive PCR results and patients' gender. IgM presence was observed in 11% (6.51) of cases and 6.5% (2.31) of controls (P = 0.7). IgG results were not positive in any of the cases (0.51), whereas positive results were seen in 13.3% of the controls (P = 0.007). IgM results showed no significant difference with PCR results (P = 0.7) but IgG results did (P = 0.05). CONCLUSIONS: The results of the study showed a positive correlation between PCR and serology (IgM) results in the case group. Recent C. pneumoniae infection was proved to exist by PCR and serology (IgM) in patients who had undergone adenoidectomy. In contrast to the controls, none of the patients in the study group showed previous immunity. Findings indicate the probable role of C. pneumoniae infection, in inducing adenoid hypertrophy in nearly 13% of cases.
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spelling pubmed-42552112014-12-05 Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis Javadi Nia, Shima Zarabi, Vida Noorbakhsh, Samileh Farhadi, Mohammad Ghavidel Darestani, Sahar Jundishapur J Microbiol Research Article BACKGROUND: Since adenoids may act as a reservoir for bacteria, they can cause ear infection, recurrent otitis and recurrent adenotonsillitis. Therefore, adenotonsillectomy is an efficient method for reducing the number and severity of subsequent infections. OBJECTIVES: This study aimed to determine the Chlamydophila pneumonia infection by serological tests and performing PCR in the adenoid tissue, in children undergoing adenoid surgery and compare the results with normal children. PATIENTS AND METHODS: This case control study was conducted in the ENT and pediatric wards of Rasoul Akram Hospital in Tehran (2008-2011). We studied 53 patients who had undergone the adenoid surgery (adenoid hypertrophy concomitant with rhino sinusitis) and 31 other patients undergoing elective surgeries in the general surgery ward (like orthopedic surgeries, hernia, etc.) without any infection (like rhinosinusitis), as the control groups. We searched for C. pneumoniae by PCR kits (Chemicon, Germany) in resected adenoid tissues and nasopharyngeal swabs of controls (ethics restrictions in controls). We also looked for specific IgM & IgG antibodies (ELISA, Biochem Immuno Systems, Italy) and compared them between the two groups. A P value < 0.05 was considered statistically significant. RESULTS: Positive PCR results were observed in 13.5% of cases and none of the controls. The condition prevalence was higher in warm seasons (P = 0.05). No correlation was found between positive PCR results and patients' gender. IgM presence was observed in 11% (6.51) of cases and 6.5% (2.31) of controls (P = 0.7). IgG results were not positive in any of the cases (0.51), whereas positive results were seen in 13.3% of the controls (P = 0.007). IgM results showed no significant difference with PCR results (P = 0.7) but IgG results did (P = 0.05). CONCLUSIONS: The results of the study showed a positive correlation between PCR and serology (IgM) results in the case group. Recent C. pneumoniae infection was proved to exist by PCR and serology (IgM) in patients who had undergone adenoidectomy. In contrast to the controls, none of the patients in the study group showed previous immunity. Findings indicate the probable role of C. pneumoniae infection, in inducing adenoid hypertrophy in nearly 13% of cases. Kowsar 2014-07-01 2014-08 /pmc/articles/PMC4255211/ /pubmed/25485049 http://dx.doi.org/10.5812/jjm.11134 Text en Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Javadi Nia, Shima
Zarabi, Vida
Noorbakhsh, Samileh
Farhadi, Mohammad
Ghavidel Darestani, Sahar
Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis
title Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis
title_full Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis
title_fullStr Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis
title_full_unstemmed Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis
title_short Chlamydophila pneumoniae Infection Assessment in Children With Adenoid Hypertrophy Concomitant With Rhino Sinusitis
title_sort chlamydophila pneumoniae infection assessment in children with adenoid hypertrophy concomitant with rhino sinusitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255211/
https://www.ncbi.nlm.nih.gov/pubmed/25485049
http://dx.doi.org/10.5812/jjm.11134
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